SYBR Green qPCR primers for detecting human ATIC (5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human ATIC qPCR Primer Set – 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
Also known as: AICAR, AICARFT, HEL-S-70p, IMPCHASE, PURH
About this gene: ATIC encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in ATIC leads to AICA-ribosiduria.
Gene details: NCBI Gene ID: 471; HGNC ID: HGNC:794; UniProt: P31939; RefSeq (MANE Select): NM_004044.7; Chromosome location: 2q35
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.