SYBR Green qPCR primers for detecting human ASF1A (anti-silencing function 1A histone chaperone, also called CIA) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human ASF1A qPCR Primer Set – anti-silencing function 1A histone chaperone
Also known as: CGI-98, CIA, HSPC146
About this gene: ASF1A belongs to the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. The ASF1A gene product interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair.
Gene details: NCBI Gene ID: 25842; HGNC ID: HGNC:20995; UniProt: Q9Y294; RefSeq (MANE Select): NM_014034.3; Chromosome location: 6q22.31
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.