SYBR Green qPCR primers for detecting human ASAH1 (N-acylsphingosine amidohydrolase 1, also called AC) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human ASAH1 qPCR Primer Set – N-acylsphingosine amidohydrolase 1
Also known as: AC, ACDase, ASAH, PHP, PHP32, SMAPME
About this gene: ASAH1 belongs to the acid ceramidase family of proteins. Processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression.
Gene details: NCBI Gene ID: 427; HGNC ID: HGNC:735; UniProt: Q13510; RefSeq (MANE Select): NM_177924.5; Chromosome location: 8p22
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.