SYBR Green qPCR primers for detecting human APOB (apolipoprotein B) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human APOB qPCR Primer Set – apolipoprotein B
Also known as: FCHL2, FLDB, LDLCQ4, apoB-100, apoB-48
About this gene: APOB product is the main apolipoprotein of chylomicrons and low density lipoproteins, and is the ligand for the LDL receptor. The APOB gene product occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence.
Gene details: NCBI Gene ID: 338; HGNC ID: HGNC:603; UniProt: P04114; RefSeq (MANE Select): NM_000384.3; Chromosome location: 2p24.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.