SYBR Green qPCR primers for detecting human CFI (complement factor I) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human CFI qPCR Primer Set – complement factor I
Also known as: AHUS3, ARMD13, C3BINA, C3b-INA, FI, IF, KAF
About this gene: CFI encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes.
Gene details: NCBI Gene ID: 3426; HGNC ID: HGNC:5394; UniProt: P05156; RefSeq (MANE Select): NM_000204.5; Chromosome location: 4q25
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.