SYBR Green qPCR primers for detecting human ALAS1 (5'-aminolevulinate synthase 1, also called ALAS-H) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human ALAS1 qPCR Primer Set – 5'-aminolevulinate synthase 1
Also known as: ALAS, ALAS-H, ALAS3, ALASH, MIG4
About this gene: ALAS1 encodes a mitochondrial enzyme which is catalyzes the rate-limiting step in heme biosynthesis. The enzyme encoded by ALAS1 is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate.
Gene details: NCBI Gene ID: 211; HGNC ID: HGNC:396; UniProt: P13196; RefSeq (MANE Select): NM_000688.6; Chromosome location: 3p21.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.