SYBR Green qPCR primers for detecting human ASAP2 (ArfGAP with SH3 domain, ankyrin repeat and PH domain 2, also called PAP) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human ASAP2 qPCR Primer Set – ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
Also known as: AMAP2, CENTB3, DDEF2, PAG3, PAP, Pap-alpha, SHAG1
About this gene: ASAP2 encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta. The ASAP2 protein forms a stable complex with PYK2 in vivo.
Gene details: NCBI Gene ID: 8853; HGNC ID: HGNC:2721; UniProt: O43150; RefSeq (MANE Select): NM_003887.3; Chromosome location: 2p25.1|2p24
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.