SYBR Green qPCR primers for detecting human COL6A1 (collagen type VI alpha 1 chain, also called VI) gene expression by real-time PCR.
Human COL6A1 qPCR Primer Set – collagen type VI alpha 1 chain
Also known as: BTHLM1, BTHLM1A, OPLL, UCHMD1, UCHMD1A
About this gene: The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils.
Gene details: NCBI Gene ID: 1291; HGNC ID: HGNC:2211; UniProt: P12109; RefSeq (MANE Select): NM_001848.3; Chromosome location: 21q22.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.