SYBR Green qPCR primers for detecting human CHFR (checkpoint with forkhead and ring finger domains) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human CHFR qPCR Primer Set – checkpoint with forkhead and ring finger domains
Also known as: RNF116, RNF196
About this gene: CHFR encodes a E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The CHFR protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region.
Gene details: NCBI Gene ID: 55743; HGNC ID: HGNC:20455; UniProt: Q96EP1; RefSeq (MANE Select): NM_001161346.2; Chromosome location: 12q24.33
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.