SYBR Green qPCR primers for detecting human CEP250 (centrosomal protein 250, also called C-Nap1) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human CEP250 qPCR Primer Set – centrosomal protein 250
Also known as: C-NAP1, CEP2, CNAP1, CRDHL2
About this gene: CEP250 encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The CEP250 protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also linked to the centrosome.
Gene details: NCBI Gene ID: 11190; HGNC ID: HGNC:1859; UniProt: Q9BV73; RefSeq (MANE Select): NM_007186.6; Chromosome location: 20q11.22
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.