SYBR Green qPCR primers for detecting human CENPA (centromere protein A, also called CENP-A) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human CENPA qPCR Primer Set – centromere protein A
Also known as: CENP-A, CenH3
About this gene: Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the2 tetrameric core of the nucleosome particle.
Gene details: NCBI Gene ID: 1058; HGNC ID: HGNC:1851; UniProt: P49450; RefSeq (MANE Select): NM_001809.4; Chromosome location: 2p23.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.