SYBR Green qPCR primers for detecting human CDC27 (cell division cycle 27, also called APC3) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human CDC27 qPCR Primer Set – cell division cycle 27
Also known as: ANAPC3, APC3, CDC27Hs, D0S1430E, D17S978E, H-NUC, HNUC, NUC2
About this gene: The CDC27 protein shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. The CDC27 protein is a component of the anaphase-promoting complex, which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins.
Gene details: NCBI Gene ID: 996; HGNC ID: HGNC:1728; UniProt: P30260; RefSeq (MANE Select): NM_001256.6; Chromosome location: 17q21.32
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.