SYBR Green qPCR primers for detecting human NDUFAF5 (NADH:ubiquinone oxidoreductase complex assembly factor 5) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human NDUFAF5 qPCR Primer Set – NADH:ubiquinone oxidoreductase complex assembly factor 5
Also known as: C20orf7, MC1DN16, bA526K24.2, dJ842G6.1
About this gene: The NADH-ubiquinone oxidoreductase complex of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. NDUFAF5 encodes a mitochondrial protein that is linked to the matrix face of the mitochondrial inner membrane and is required for complex I assembly. A mutation in NDUFAF5 leads to mitochondrial complex I deficiency.
Gene details: NCBI Gene ID: 79133; HGNC ID: HGNC:15899; UniProt: Q5TEU4; RefSeq (MANE Select): NM_024120.5; Chromosome location: 20p12.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.