SYBR Green qPCR primers for detecting human SHFL (shiftless antiviral inhibitor of ribosomal frameshifting, also called SFL) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human SHFL qPCR Primer Set – shiftless antiviral inhibitor of ribosomal frameshifting
Also known as: C19orf66, IRAV, RyDEN, SFL
About this gene: SHFL is an interferon stimulated gene that inhibits viral replication. The SHFL protein binds nucleic acids and inhibits programmed -1 ribosomal frameshifting required for translation by many RNA viruses. Viruses inhibited by the protein include Zika virus, dengue virus and the coronaviruses, SARS-CoV and SARS-CoV2.
Gene details: NCBI Gene ID: 55337; HGNC ID: HGNC:25649; UniProt: Q9NUL5; RefSeq (MANE Select): NM_018381.4; Chromosome location: 19p13.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.