SYBR Green qPCR primers for detecting human ACTB (actin beta, also called beta-actin) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human ACTB qPCR Primer Set – actin beta
Also known as: BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
About this gene: ACTB encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The ACTB protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed.
Gene details: NCBI Gene ID: 60; HGNC ID: HGNC:132; UniProt: P60709; RefSeq (MANE Select): NM_001101.5; Chromosome location: 7p22.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets, or profile ACTB alongside related genes with our Housekeeping Gene Primer Sets.