Ubiquitination is the attachment of the small protein ubiquitin to target proteins, which marks them for degradation by the proteasome or changes their activity, location or interactions. Ubiquitin is activated by an E1 enzyme, transferred to an E2 conjugating enzyme, and attached to the substrate by one of hundreds of E3 ubiquitin ligases (such as RING, HECT and cullin-based ligases) that provide substrate specificity. Deubiquitinating enzymes (DUBs) remove ubiquitin. The ubiquitin-proteasome system controls protein quality, the cell cycle, DNA repair, signaling and immunity, and is implicated in cancer and neurodegeneration.
A ready-to-use Rat Ubiquitin Metabolism PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against ubiquitin metabolism genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: Anapc2 anaphase promoting complex subunit 2 Anapc11 anaphase promoting complex subunit 11 Arih1 ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1 Atg7 ATG7 autophagy related 7 homolog Bard1 BRCA1 associated RING domain 1 Brca1 Breast cancer 1, early onset Brca2 Breast cancer 2, early onset Brcc3 BRCA1/BRCA2-containing complex, subunit 3 Btrc beta-transducin repeat containing Cbl Cas-Br-M (murine) ecotropic retroviral transforming sequence Cdc34 cell division cycle 34 homolog Cul1 cullin 1 Cul2 cullin 2 Cul3 cullin 3 Cul4b cullin 4B Cul5 cullin 5 Ddb1 damage-specific DNA binding protein 1, 127kDa Fbxo11 F-box protein 11 Fbxo32 F-box protein 32 Fbxo4 F-box protein 4 Fbxo6 F-box protein 6 Fbxw9 F-box and WD repeat domain containing 9 Hecw2 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 Itch itchy E3 ubiquitin protein ligase homolog (mouse) March5 membrane-associated ring finger (C3HC4) 5 Mdm2 Mdm2 p53 binding protein homolog Mib1 mindbomb homolog 1 (Drosophila) Mocs3 molybdenum cofactor synthesis 3 Mul1 mitochondrial E3 ubiquitin protein ligase 1 Nae1 NEDD8 activating enzyme E1 subunit 1 Nedd8 neural precursor cell expressed, developmentally down-regulated 8 Park2 Parkinson disease (autosomal recessive, juvenile) 2, parkin Rfwd2 ring finger and WD repeat domain 2 Rlim ring finger protein, LIM domain interacting Rnf144b ring finger protein 144B Rnf167 ring finger protein 167 Rnf180 ring finger protein 180 Rnf2 ring finger protein 2 Rnf20 ring finger protein 20 Rnf34 ring finger protein 34 Rnf40 ring finger protein 40 Rnf6 ring finger protein (C3H2C3 type) 6 Rnf8 ring finger protein 8 Sae1 SUMO1 activating enzyme subunit 1 Skp1 S-phase kinase-associated protein 1 Skp2 S-phase kinase-associated protein 2 (p45) Smurf1 SMAD specific E3 ubiquitin protein ligase 1 Smurf2 SMAD specific E3 ubiquitin protein ligase 2 Stub1 STIP1 homology and U-Box containing protein 1 Syvn1 synovial apoptosis inhibitor 1, synoviolin Tmem189 transmembrane protein 189 Tp53 tumor protein p53 Uba1 ubiquitin-like modifier activating enzyme 1 Uba2 ubiquitin-like modifier activating enzyme 2 Uba3 ubiquitin-like modifier activating enzyme 3 Uba5 ubiquitin-like modifier activating enzyme 5 Uba6 ubiquitin-like modifier activating enzyme 6 Ube2a ubiquitin-conjugating enzyme E2A Ube2b ubiquitin-conjugating enzyme E2B Ube2c ubiquitin-conjugating enzyme E2C Ube2d1 ubiquitin-conjugating enzyme E2D 1 Ube2d3 ubiquitin-conjugating enzyme E2D 3 Ube2e2 ubiquitin-conjugating enzyme E2E 2 Ube2e3 ubiquitin-conjugating enzyme E2E 3 Ube2g1 ubiquitin-conjugating enzyme E2G 1 Ube2g2 ubiquitin-conjugating enzyme E2G 2 Ube2i ubiquitin-conjugating enzyme E2I Ube2j1 ubiquitin-conjugating enzyme E2, J1 Ube2j2 ubiquitin-conjugating enzyme E2, J2 Ube2k ubiquitin-conjugating enzyme E2K Ube2l3 ubiquitin-conjugating enzyme E2L 3 Ube2l6 ubiquitin-conjugating enzyme E2L 6 Ube2m ubiquitin-conjugating enzyme E2M Ube2n ubiquitin-conjugating enzyme E2N Ube2q1 ubiquitin-conjugating enzyme E2Q family member 1 Ube2q2 ubiquitin-conjugating enzyme E2Q family member 2 Ube2q2l ubiquitin-conjugating enzyme E2Q family member 2-like Ube2s ubiquitin-conjugating enzyme E2S Ube2t ubiquitin-conjugating enzyme E2T (putative) Ube2v1 ubiquitin-conjugating enzyme E2 variant 1 Ube2v2 ubiquitin-conjugating enzyme E2 variant 2 Ube2z ubiquitin-conjugating enzyme E2Z Ube4a ubiquitination factor E4A Uchl1 ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) Uchl3 ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) Uchl5 ubiquitin carboxyl-terminal hydrolase L5 Vhl von Hippel-Lindau tumor suppressor Wwp1 WW domain containing E3 ubiquitin protein ligase 1 Actb Actin, beta B2m Beta-2-microglobulin Gapd Glyceraldehyde-3-phosphate dehydrogenase Gusb Glucuronidase, beta Hprt1 Hypoxanthine phosphoribosyltransferase 1 Pgk Phosphoglycerate kinase 1 Ppia Peptidylprolyl isomerase A Rpl13a Ribosomal protein L13a $595.00 |