Tumor necrosis factor (TNF) is a major pro-inflammatory cytokine produced mainly by macrophages and T cells. TNF binds two receptors, TNFRSF1A (TNFR1) and TNFRSF1B (TNFR2). TNFR1 recruits TRADD, RIPK1, TRAF2 and cIAPs to form a signaling complex that activates NF-kappaB and MAPK pathways and induces inflammatory genes. Under certain conditions, TNFR1 instead forms complexes with FADD and caspase 8 that trigger apoptosis, or with RIPK3 and MLKL that trigger necroptosis. TNF signaling drives inflammatory diseases such as rheumatoid arthritis and inflammatory bowel disease, which are treated with TNF inhibitors.
A ready-to-use Human TNF Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against TNF signaling genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: AKT1 v-akt murine thymoma viral oncogene homolog 1BAD BCL2-associated agonist of cell death BAX BCL2-associated X protein BCL2L1 BCL2-like BID BH3 intercting domain death agonist BIRC2 baculovial IAP repeat containing 2 BIRC3 baculovral IAP repeat containing 3 BTRC beta-transducin repeat containing E3 ubiquitin protein ligase CASP7 caspase 7, apoptosis-related cysteine peptidase CASP8 caspase 8, apoptosis-related cysteine peptidase CASP9 caspase 9, apoptosis-related cysteine peptidase CD27 CD27 molecule CD40 CD40 molecule, TNF receptor superfamily member 5 CD40LG CD40 ligand CD70 CD70 molecule CFLAR CASP8 and FADD-like apoptosis regulator CHUK conserved helix-loop-helix ubiquitous kinase CREBBP CREB binding protein CSNK2A1 casein kinase 2, alpha 1 polypeptide CUL1 cullin 1 CYBA cytochrome b-245, alpha polypeptide EDA2R ectodysplasin A2 receptor FADD Fas (TNFRSF6)-associated via death domain FAS Fas (TNF receptor superfamily, member 6) FASLG Fas ligand (TNF superfamily, member 6) GLUL glutamate-ammonia ligase GRB2 growth factor receptor-bound protein 2 IKBKB inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta IKBKG inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma JUN jun proto-oncogene LTA lymphotoxin alpha (TNF superfamily, member 1) LTB lymphotoxin beta (TNF superfamily, member 3) LTBR lymphotoxin beta receptor (TNFR superfamily, member 3) MADD MAP-kinase activating death domain MAP2K4 mitogen-activated protein kinase kinase 4 MAP2K6 mitogen-activated protein kinase kinase 6 MAP3K1 mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase MAP3K3 mitogen-activated protein kinase kinase kinase 3 MAP3K5 mitogen-activated protein kinase kinase kinase 5 MAP3K7 mitogen-activated protein kinase kinase kinase 7 MAP3K8 mitogen-activated protein kinase kinase kinase 8 MAPK1 mitogen-activated protein kinase 1 MAPK3 mitogen-activated protein kinase 3 MAPK8 mitogen-activated protein kinase 8 NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 NFKBIA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha NFKBIB nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta NFKBIE nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon NGFR nerve growth factor receptor NOX1 NADPH oxidase 1 NOXO1 NADPH oxidase organizer 1 PLAD Data not found PLK1 polo-like kinase 1 PPP2CA protein phosphatase 2, catalytic subunit, alpha isozyme PRKCZ protein kinase C, zeta PSMD2 proteasome (prosome, macropain) 26S subunit, non- ATPase, 2 PYGL phosphorylase, glycogen, liver RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) RAS Data not found RFK riboflavin kinase RIPK1 receptor (TNFRSF)-interacting serine-threonine kinase 1 RIPK3 receptor-interacting serine-threonine kinase 3 SKP1 S-phase kinase-associated protein 1 SOS1 son of sevenless homolog 1 (Drosophila) TAB1 TGF-beta activated kinase 1/MAP3K7 binding protein 1 TAB2 TGF-beta activated kinase 1/MAP3K7 binding protein 2 TAB3 TGF-beta activated kinase 1/MAP3K7 binding protein 3 TANK TRAF family member-associated NFKB activator TBK1 TANK-binding kinase 1 TNF tumor necrosis factor TNFAIP3 tumor necrosis factor, alpha-induced protein 3 TNFRSF10A tumor necrosis factor receptor superfamily, member 10a TNFRSF10B tumor necrosis factor receptor superfamily, member 10b TNFRSF1A tumor necrosis factor receptor superfamily, member 1A TNFRSF1B tumor necrosis factor receptor superfamily, member 1B TNFRSF4 tumor necrosis factor receptor superfamily, member 4 TNFRSF8 tumor necrosis factor receptor superfamily, member 8 TNFRSF9 tumor necrosis factor receptor superfamily, member 9 TNFSF10 tumor necrosis factor (ligand) superfamily, member 10 TNFSF11 tumor necrosis factor (ligand) superfamily, member 11 TNFSF12 tumor necrosis factor (ligand) superfamily, member 12 TNFSF13 tumor necrosis factor (ligand) superfamily, member 13 TNFSF13B tumor necrosis factor (ligand) superfamily, member 13b TRADD TNFRSF1A-associated via death domain TRAF1 TNF receptor-associated factor 1 TRAF2 TNF receptor-associated factor 2 TRAF3 TNF receptor-associated factor 3 TRAP1 TNF receptor-associated protein 1 ACTB Actin, beta B2M Beta-2-microglobulin GAPDH Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK1 Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $495.00 |