After activation, naive CD4+ T helper cells differentiate into specialized subsets depending on the cytokines present. IL-12 and IFN-gamma signaling through STAT4 and STAT1 induce the transcription factor TBX21 (T-bet) and Th1 cells, which produce IFN-gamma and defend against intracellular pathogens. IL-4 signaling through STAT6 induces GATA3 and Th2 cells, which produce IL-4, IL-5 and IL-13 and defend against parasites but also drive allergy and asthma. Th1 and Th2 programs inhibit each other, and the balance between them shapes immune responses in infection, allergy and autoimmune disease.
A ready-to-use Human Th1 and Th2 Cell Differentiation PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against Th1 and Th2 differeniation genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: CD247 CD247 molecule CD3D CD3d molecule, delta (CD3-TCR complex) CD3E CD3e molecule, epsilon (CD3-TCR complex) CD3G CD3g molecule, gamma (CD3-TCR complex) CD4 CD4 molecule CHUK conserved helix-loop-helix ubiquitous kinase DLL1 delta-like 1 (Drosophila) DLL3 delta-like 3 (Drosophila) DLL4 delta-like 4 (Drosophila) FOS FBJ murine osteosarcoma viral oncogene homolog GATA3 GATA binding protein 3 HLA-DMA major histocompatibility complex, class II, DM alpha HLA-DMB major histocompatibility complex, class II, DM beta HLA-DOA major histocompatibility complex, class II, DO alpha HLA-DOB major histocompatibility complex, class II, DO beta HLA-DPA1 major histocompatibility complex, class II, DP alpha 1 HLA-DPB1 major histocompatibility complex, class II, DP beta 1 HLA-DQA1 major histocompatibility complex, class II, DQ alpha 1 HLA-DQA2 major histocompatibility complex, class II, DQ alpha 2 HLA-DQB1 major histocompatibility complex, class II, DQ beta 1 HLA-DRA major histocompatibility complex, class II, DR alpha HLA-DRB1 major histocompatibility complex, class II, DR beta 1 HLA-DRB3 major histocompatibility complex, class II, DR beta 3 HLA-DRB4 major histocompatibility complex, class II, DR beta 4 HLA-DRB5 major histocompatibility complex, class II, DR beta 5 IFNG interferon, gamma IFNGR1 interferon gamma receptor 1 IFNGR2 interferon gamma receptor 2 (interferon gamma transducer 1) IKBKB inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta IKBKG inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma IL12A interleukin 12A IL12B interleukin 12B IL12RB1 interleukin 12 receptor, beta 1 IL12RB2 interleukin 12 receptor, beta 2 IL13 interleukin 13 IL2 interleukin 2 IL2RA interleukin 2 receptor, alpha IL2RB interleukin 2 receptor, beta IL2RG interleukin 2 receptor, gamma IL4 interleukin 4 IL4R interleukin 4 receptor IL5 interleukin 5 JAK1 Janus kinase 1 JAK2 Janus kinase 2 JAK3 Janus kinase 3 JUN jun proto-oncogene LAT linker for activation of T cells LCK LCK proto-oncogene, Src family tyrosine kinase MAF v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog MAML1 mastermind-like 1 (Drosophila) MAML2 mastermind-like 2 (Drosophila) MAML3 mastermind-like 3 (Drosophila) MAPK1 mitogen-activated protein kinase 1 MAPK10 mitogen-activated protein kinase 10 MAPK11 mitogen-activated protein kinase 11 MAPK12 mitogen-activated protein kinase 12 MAPK13 mitogen-activated protein kinase 13 MAPK14 mitogen-activated protein kinase 14 MAPK3 mitogen-activated protein kinase 3 MAPK8 mitogen-activated protein kinase 8 MAPK9 mitogen-activated protein kinase 9 NFATC1 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 NFATC2 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 NFKBIA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha NFKBIB nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta NFKBIE nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon NOTCH1 notch 1 NOTCH2 notch 2 NOTCH3 notch 3 PLCG1 phospholipase C, gamma 1 PLCG2 phospholipase C, gamma 2 (phosphatidylinositol-specific) PPP3CA protein phosphatase 3, catalytic subunit, alpha isozyme PPP3CB protein phosphatase 3, catalytic subunit, beta isozyme PPP3CC protein phosphatase 3, catalytic subunit, gamma isozyme PPP3R1 protein phosphatase 3, regulatory subunit B, alpha PPP3R2 protein phosphatase 3, regulatory subunit B, beta PRKCQ protein kinase C, theta RBPJ recombination signal binding protein for immunoglobulin kappa J region RBPJL recombination signal binding protein for immunoglobulin kappa J region- like RELA v-rel avian reticuloendotheliosis viral oncogene homolog A RUNX3 runt-related transcription factor 3 STAT1 signal transducer and activator of transcription 1, 91kDa STAT4 signal transducer and activator of transcription 4 STAT6 signal transducer and activator of transcription 6, interleukin-4 induced TBX21 T-box 21 TYK2 tyrosine kinase 2 ZAP70 zeta-chain (TCR) associated protein kinase 70kDa ACTB Actin, beta B2M Beta-2-microglobulin GAPDH Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK1 Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $495.00 |