RNase R is a 3' to 5' exonuclease closely related to RNase II, which has been shown to be involved in selective mRNA degradation, particularly of non stop mRNAs in bacteria.
RNase R has homologues in many other organisms. This enzyme has been used by researchers to selectively enrich an RNA population for circRNA. This enzyme is supplied in 50 units and includes a 10X reaction buffer.
Buffer: RNase R is supplied in a 50% glycerol solution containing 50 mM Tris-HCl (7.5), 100 mM NaCl, O.1 mM EDTA, O.1% Triton X-100 and 1 mM dithiothreitol.
Activity: 20 U/ul
Concentration: 1 ug/ul
Unit Definition: One Unit converts 1 ug of poly-r(A) into acid-soluble nucleotides in 10 min at 37C in 20 mM Tris-HCl (pH 8.0), 100 mM KCl and 0.1 mM MgCl2.