The PI3K-AKT pathway is a major signaling pathway that controls cell growth, survival, metabolism and proliferation. Receptor tyrosine kinases and G protein coupled receptors activate phosphoinositide 3-kinase (PI3K), which converts PIP2 to PIP3 at the cell membrane. PIP3 recruits AKT and PDK1, and activated AKT phosphorylates targets such as GSK3, FOXO transcription factors, BAD and TSC2, leading to activation of mTOR and protein synthesis. The tumor suppressor PTEN turns the pathway off. PI3K-AKT signaling is one of the most frequently activated pathways in cancer and is also central to insulin action.
A ready-to-use Human PI3K-AKT Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against PI3K/AKT signaling genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: ABL1 c-abl oncogene 1, receptor tyrosine kinase ACACA acetyl-Coenzyme A carboxylase alpha ACOX1 acyl-Coenzyme A oxidase 1, palmitoyl AEBP1 AE binding protein 1 AKT1 v-akt murine thymoma viral oncogene homolog 1 AKT2 v-akt murine thymoma viral oncogene homolog 2 AKT3 v-akt murine thymoma viral oncogene homolog 3 ANG angiogenin, ribonuclease, RNase A family, 5 ARAF v-raf murine sarcoma 3611 viral oncogene homolog BAD BCL2-associated agonist of cell death BCL2 B-cell CLL/lymphoma 2 BCL2L1 BCL2-like 1 BRAF v-raf murine sarcoma viral oncogene homolog B1 BTK Bruton agammaglobulinemia tyrosine kinase CAP1 CAP, adenylate cyclase-associated protein 1 (yeast) CASP9 caspase 9, apoptosis-related cysteine peptidase CBL Cas-Br-M (murine) ecotropic retroviral transforming sequence CCND1 cycline D1 CCR2 chemokine (C-C motif) receptor 2 CCR5 chemokine (C-C motif) receptor 5 CD4 CD4 molecule CD40 CD40 molecule, TNF receptor superfamily member 5 CDC42 cell division cycle 42 (GTP binding protein, 25kDa) CDH1 cadherin 1, type 1, E-cadherin (epithelial) CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1) CDKN1B cyclin-dependent kinase inhibitor 1B (p27, Kip1) CDKN2A cyclin-dependent kinase inhibitor 2A (p16) CREB1 cAMP responsive element binding protein 1 CTNNB1 catenin (cadherin-associated protein), beta 1, 88kDa CXCR4 chemokine (C-X-C motif) receptor 4 E2F1 E2F transcription factor 1 EGFR epidermal growth factor receptor ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 FRAP1 Fkbp12-Rapamycin Complex-Associated Protein 1 GRB2 growth factor receptor-bound protein 2 GSK3A glycogen synthase kinase 3 alpha GSK3B glycogen synthase kinase 3 beta GYS1 glycogen synthase 1 (muscle) HK2 hexokinase 2 HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog IGF1 insulin-like growth factor 1 (somatomedin C) IGF1R insulin-like growth factor 1 receptor IGF2 insulin-like growth factor 2 (somatomedin A) IGF2R insulin-like growth factor 2 receptor INS insulin INSR insulin receptor IRS1 insulin receptor substrate 1 IRS2 insulin receptor substrate 2 IRS4 insulin receptor substrate 4 ITGB1 integrin, beta 1 ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) JAK2 Janus kinase 2 KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog LCK lymphocyte-specific protein tyrosine kinase LEP leptin MAP2K1 mitogen-activated protein kinase kinase 1 MAPK1 mitogen-activated protein kinase 1 MAPK14 mitogen-activated protein kinase 14 MAPK8 mitogen-activated protein kinase 8 MYC v-myc myelocytomatosis viral oncogene homolog (avian) NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 NFKBIA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha NOS3 nitric oxide synthase 3 (endothelial cell) PAK1 p21 protein (Cdc42/Rac)-activated kinase 1 PDE3B phosphodiesterase 3B, cGMP-inhibited PDPK1 3-phosphoinositide dependent protein kinase-1 PIK3C2B phosphoinositide-3-kinase, class 2, beta polypeptide PIK3C3 phosphoinositide-3-kinase, class 3 PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) PIK3R4 phosphoinositide-3-kinase, regulatory subunit 4 PRKCA protein kinase C, alpha PRKCE protein kinase C, epsilon PRKCG protein kinase C, gamma PTEN phosphatase and tensin homolog RAC1 ras-related C3 botulinum toxin substrate 1 RAF1 v-raf-1 murine leukemia viral oncogene homolog 1 RELA v-rel reticuloendotheliosis viral oncogene homolog A (avian) RHOA ras homolog gene family, member A SGK1 serum/glucocorticoid regulated kinase 1 SHC1 SHC (Src homology 2 domain containing) transforming protein 1 SOS1 son of sevenless homolog 1 (Drosophila) SPP1 secreted phosphoprotein 1 SRC v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog SYK spleen tyrosine kinase TLR4 toll-like receptor 4 TNF tumor necrosis factor (TNF superfamily, member 2) ACTB Actin, beta B2M Beta-2-microglobulin GAPD Glyceraldehyde phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK1 Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $395.00 |
A ready-to-use Mouse PI3K-AKT Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against PI3K/AKT signaling genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: Abl1 c-abl oncogene 1, receptor tyrosine kinase Acaca acetyl-Coenzyme A carboxylase alpha Acox1 acyl-Coenzyme A oxidase 1, palmitoyl Aebp1 AE binding protein 1 Akt1 v-akt murine thymoma viral oncogene homolog 1 Akt2 v-akt murine thymoma viral oncogene homolog 2 Akt3 v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) Ang angiogenin, ribonuclease, RNase A family, 5 Araf v-raf murine sarcoma 3611 viral oncogene homolog Bad BCL2-associated agonist of cell death Bcl2 B-cell CLL/lymphoma 2 Bcl2L1 BCL2-like 1 Braf v-raf murine sarcoma viral oncogene homolog B1 Btk Bruton agammaglobulinemia tyrosine kinase Cap1 CAP, adenylate cyclase-associated protein 1 (yeast) Casp9 caspase 9, apoptosis-related cysteine peptidase Cbl Cas-Br-M (murine) ecotropic retroviral transforming sequence Ccnd1 cycline D1 Ccr2 chemokine (C-C motif) receptor 2 Ccr5 chemokine (C-C motif) receptor 5 Cd4 CD4 molecule Cd40 CD40 molecule, TNF receptor superfamily member 5 Cdc42 cell division cycle 42 (GTP binding protein, 25kDa) Cdh1 cadherin 1, type 1, E-cadherin (epithelial) Cdkn1A cyclin-dependent kinase inhibitor 1A (p21, Cip1) Cdkn1B cyclin-dependent kinase inhibitor 1B (p27, Kip1) Cdkn2A cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) Creb1 cAMP responsive element binding protein 1 Ctnnb1 catenin (cadherin-associated protein), beta 1, 88kDa Cxcr4 chemokine (C-X-C motif) receptor 4 E2F1 E2F transcription factor 1 Egfr epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) Erbb2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) Frap1 FKBP12-RAPAMYCIN COMPLEX-ASSOCIATED PROTEIN 1 Grb2 growth factor receptor-bound protein 2 Gsk3A glycogen synthase kinase 3 alpha Gsk3B glycogen synthase kinase 3 beta Gys1 glycogen synthase 1 (muscle) Hk2 hexokinase 2 Hras v-Ha-ras Harvey rat sarcoma viral oncogene homolog Igf1 insulin-like growth factor 1 (somatomedin C) Igf1R insulin-like growth factor 1 receptor Igf2 insulin-like growth factor 2 (somatomedin A) Igf2R insulin-like growth factor 2 receptor Ins insulin Insr insulin receptor Irs1 insulin receptor substrate 1 Irs2 insulin receptor substrate 2 Irs4 insulin receptor substrate 4 Itgb1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) Itgb3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) Jak2 Janus kinase 2 Kit v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog Kras v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog Lck lymphocyte-specific protein tyrosine kinase Lep leptin Map2K1 mitogen-activated protein kinase kinase 1 Mapk1 mitogen-activated protein kinase 1 Mapk14 mitogen-activated protein kinase 14 Mapk8 mitogen-activated protein kinase 8 Myc v-myc myelocytomatosis viral oncogene homolog (avian) Nfkb1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 Nfkbia nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha Nos3 nitric oxide synthase 3 (endothelial cell) Pak1 p21 protein (Cdc42/Rac)-activated kinase 1 Pde3B phosphodiesterase 3B, cGMP-inhibited Pdpk1 3-phosphoinositide dependent protein kinase-1 Pik3C2B phosphoinositide-3-kinase, class 2, beta polypeptide Pik3C3 phosphoinositide-3-kinase, class 3 Pik3Ca phosphoinositide-3-kinase, catalytic, alpha polypeptide Pik3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) Pik3R4 phosphoinositide-3-kinase, regulatory subunit 4 Prkca protein kinase C, alpha Prkce protein kinase C, epsilon Prkcg protein kinase C, gamma Pten phosphatase and tensin homolog Rac1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) Raf1 v-raf-1 murine leukemia viral oncogene homolog 1 Rela v-rel reticuloendotheliosis viral oncogene homolog A (avian) Rhoa ras homolog gene family, member A Sgk1 serum/glucocorticoid regulated kinase 1 Shc1 SHC (Src homology 2 domain containing) transforming protein 1 Sos1 son of sevenless homolog 1 (Drosophila) Spp1 secreted phosphoprotein 1 Src v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) Syk spleen tyrosine kinase Tlr4 toll-like receptor 4 Tnf tumor necrosis factor (TNF superfamily, member 2) Actb actin, beta B2M beta-2-microglobulin Gapdh glyceraldehyde-phosphate-dehydrogenase Gusb glucuronidase, beta Hprt1 hypoxanthine phosphoribosyltransferase 1 Pgk1 phosphoglycerate kinase 1 Ppia peptidylprolyl isomerase A (cyclophilin A) Rpl13A ribosomal protein L13a $495.00 |