Nitric oxide (NO) is a gaseous signaling molecule produced from L-arginine by three nitric oxide synthases: neuronal NOS1, inducible NOS2 and endothelial NOS3. NO activates soluble guanylate cyclase to produce cyclic GMP, which activates protein kinase G (PKG) to relax smooth muscle, dilate blood vessels and inhibit platelet aggregation. NO also acts as a neurotransmitter and, at the high levels produced by NOS2 in macrophages, as an antimicrobial and cytotoxic agent. Disrupted NO signaling is involved in hypertension, atherosclerosis, erectile dysfunction and inflammation.
A ready-to-use Human Nitric Oxide Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against nitric oxide signaling genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix Gene List: ACTA1 actin, alpha 1, skeletal muscle ACTA2 actin, alpha 2, smooth muscle, aorta ACTB actin, beta ACTG1 actin gamma 1 ACTG2 actin, gamma 2, smooth muscle, enteric ADCY1 adenylate cyclase 1 (brain) ADCY3 adenylate cyclase 3 ADCY5 adenylate cyclase 5 ADCY6 adenylate cyclase 6 ADCY7 adenylate cyclase 7 AKT1 v-akt murine thymoma viral oncogene homolog 1 AQP1 aquaporin 1 (Colton blood group) AQP2 aquaporin 2 (collecting duct) AQP3 aquaporin 3 (Gill blood group) AQP4 aquaporin 4 AQP5 aquaporin 5 BDKRB1 bradykinin receptor B1 BDKRB2 bradykinin receptor B2 CALM1 calmodulin 1 (phosphorylase kinase, delta) CALM2 calmodulin 2 (phosphorylase kinase, delta) CALM3 calmodulin 3 (phosphorylase kinase, delta) CASP3 caspase 3, apoptosis-related cysteine peptidase CASP8 caspase 8, apoptosis-related cysteine peptidase CAV1 caveolin 1, caveolae protein, 22kDa CCNA1 cyclin A1 CCNA2 cyclin A2 CNGA1 cyclic nucleotide gated channel alpha 1 CNGA3 cyclic nucleotide gated channel alpha 3 CNGB1 cyclic nucleotide gated channel beta 1 CNGB3 cyclic nucleotide gated channel beta 3 DNM2 dynamin 2 EGFR epidermal growth factor receptor ERBB2 erb-b2 receptor tyrosine kinase 2 ESR1 estrogen receptor 1 ESR2 estrogen receptor 2 (ER beta) FLT1 fms-related tyrosine kinase 1 FLT4 fms-related tyrosine kinase 4 GNAI1 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 GNAI2 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 GNAI3 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 GNAS GNAS complex locus GNB1 guanine nucleotide binding protein (G protein), beta polypeptide 1 GNB2 guanine nucleotide binding protein (G protein), beta polypeptide 2 GNB3 guanine nucleotide binding protein (G protein), beta polypeptide 3 GNB5 guanine nucleotide binding protein (G protein), beta 5 GNG2 guanine nucleotide binding protein (G protein), gamma 2 GUCY2C guanylate cyclase 2C GUCY2D guanylate cyclase 2D, membrane (retina-specific) HSP90AA1 heat shock protein 90kDa alpha (cytosolic), class A member 1 HSPA4 heat shock 70kDa protein 4 IGF1 insulin-like growth factor 1 (somatomedin C) IGF1R insulin-like growth factor 1 receptor IL2 interleukin 2 IL6 interleukin 6 ITPR1 inositol 1,4,5-trisphosphate receptor, type 1 ITPR2 inositol 1,4,5-trisphosphate receptor, type 2 ITPR3 inositol 1,4,5-trisphosphate receptor, type 3 KDR kinase insert domain receptor KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog LPAR1 lysophosphatidic acid receptor 1 LPAR2 lysophosphatidic acid receptor 2 NOS3 nitric oxide synthase 3 (endothelial cell) NOSIP nitric oxide synthase interacting protein NOSTRIN nitric oxide synthase trafficking NPR1 natriuretic peptide receptor 1 PDK1 pyruvate dehydrogenase kinase, isozyme 1 PDK2 pyruvate dehydrogenase kinase, isozyme 2 PDK3 pyruvate dehydrogenase kinase, isozyme 3 PDK4 pyruvate dehydrogenase kinase, isozyme 4 PIK3CA phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha PIK3CB phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta PIK3CD phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit delta PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) PIK3R2 phosphoinositide-3-kinase, regulatory subunit 2 (beta) PLCG1 phospholipase C, gamma 1 PLCG2 phospholipase C, gamma 2 (phosphatidylinositol-specific) PRKACA protein kinase, cAMP-dependent, catalytic, alpha PRKACB protein kinase, cAMP-dependent, catalytic, beta PRKAR1A protein kinase, cAMP-dependent, regulatory, type I, alpha PRKG1 protein kinase, cGMP-dependent, type I S1PR1 sphingosine-1-phosphate receptor 1 S1PR2 sphingosine-1-phosphate receptor 2 S1PR3 sphingosine-1-phosphate receptor 3 SLC7A1 solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 STUB1 STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase TGFB1 transforming growth factor, beta 1 VEGFA vascular endothelial growth factor A VEGFC vascular endothelial growth factor C ACTB Actin, beta B2M Beta-2-microglobulin GAPD Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $495.00 |