Type II interferon, interferon gamma (IFN-gamma), is produced mainly by T cells and NK cells and is a key activator of macrophages and cell mediated immunity. IFN-gamma binds the IFNGR1/IFNGR2 receptor, activating JAK1 and JAK2, which phosphorylate STAT1. STAT1 dimers bind gamma-activated sequence (GAS) elements and induce genes such as IRF1, CIITA, MHC class I and II, CXCL9, CXCL10 and IDO1. IFN-gamma signaling promotes antigen presentation, Th1 responses and defense against intracellular pathogens and tumors, and is regulated by SOCS1.
A ready-to-use Human Interferon Type II Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against human interferon II signaling related genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix Gene List: AKT1 v-akt murine thymoma viral oncogene homolog 1 APOBEC3G Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G ASCC3 activating signal cointegrator 1 complex subunit 3 BST2 bone marrow stromal cell antigen 2 CALM1 calmodulin 1 (phosphorylase kinase, delta) CALM2 calmodulin 2 (phosphorylase kinase, delta) CALM3 calmodulin 3 (phosphorylase kinase, delta) CAMK2A calcium/calmodulin-dependent protein kinase II alpha CAMK2B calcium/calmodulin-dependent protein kinase II beta CAMK2D calcium/calmodulin-dependent protein kinase II delta CAMK2G calcium/calmodulin-dependent protein kinase II gamma CASP1 caspase 1, apoptosis-related cysteine peptidase CBL Cbl proto-oncogene, E3 ubiquitin protein ligase CEBPB CCAAT/enhancer binding protein (C/EBP), beta CIITA class II, major histocompatibility complex, transactivator CREBBP CREB binding protein CRKL v-crk avian sarcoma virus CT10 oncogene homolog- like CXCL10 chemokine (C-X-C motif) ligand 10 CYBB cytochrome b-245, beta polypeptide DAPK1 death-associated protein kinase 1 EIF2AK2 eukaryotic translation initiation factor 2-alpha kinase 2 EP300 E1A binding protein p300 G1P2 interferon, alpha-inducible protein (clone IFI- 15K) GBP1 guanylate binding protein 1, interferon-inducible HERC5 HECT and RLD domain containing E3 ubiquitin protein ligase 5 HERC6 HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 HIST1H4B histone cluster 1, H4b HIST1H4H histone cluster 1, H4h ICAM1 intercellular adhesion molecule 1 IFI6 interferon, alpha-inducible protein 6 IFIT2 Interferon-induced protein with tetratricopeptide repeats 2 IFITM1 interferon induced transmembrane protein 1 IFITM2 interferon induced transmembrane protein 2 IFITM3 interferon induced transmembrane protein 3 IFITM5 interferon induced transmembrane protein 5 IFNA1 interferon, alpha 1 IFNB1 interferon, beta 1, fibroblast IFNG interferon, gamma IFNGR1 interferon gamma receptor 1 IFNGR2 interferon gamma receptor 2 (interferon gamma transducer 1) IL1B interleukin 1, beta IRF1 interferon regulatory factor 1 IRF2 interferon regulatory factor 2 IRF4 interferon regulatory factor 4 IRF7 interferon regulatory factor 7 IRF8 interferon regulatory factor 8 IRF9 interferon regulatory factor 9 ISG15 ISG15 ubiquitin-like modifier ISG20 interferon stimulated exonuclease gene 20kDa JAK1 Janus kinase 1 JAK2 Janus kinase 2 MAP2K1 mitogen-activated protein kinase kinase 1 MAP2K6 mitogen-activated protein kinase kinase 6 MAP3K1 mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase MAP3K11 mitogen-activated protein kinase kinase kinase 11 MAPK1 mitogen-activated protein kinase 1 MAPK3 mitogen-activated protein kinase 3 MTOR mechanistic target of rapamycin (serine/threonine kinase) MX1 MX dynamin-like GTPase 1 NOS2 nitric oxide synthase 2, inducible OAS1 2'-5'-oligoadenylate synthetase 1, 40/46kDa OASL 2'-5'-oligoadenylate synthetase-like PIAS1 protein inhibitor of activated STAT, 1 PIAS4 protein inhibitor of activated STAT, 4 PIK3CA phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) PRKCD protein kinase C, delta PSMB9 proteasome (prosome, macropain) subunit, beta type, 9 PTGES2 prostaglandin E synthase 2 PTPN11 protein tyrosine phosphatase, non-receptor type 11 RAP1A RAP1A, member of RAS oncogene family RAP1B RAP1B, member of RAS oncogene family REG1A regenerating islet-derived 1 alpha RNF31 ring finger protein 31 RSAD2 radical S-adenosyl methionine domain containing 2 SMAD7 SMAD family member 7 SOCS1 suppressor of cytokine signaling 1 SOCS3 suppressor of cytokine signaling 3 SPI1 Spi-1 proto-oncogene STAT1 signal transducer and activator of transcription 1, 91kDa STAT2 signal transducer and activator of transcription 2, 113kDa STAT3 signal transducer and activator of transcription 3 (acute-phase response factor) TAP1 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) TRIM22 tripartite motif containing 22 TRIM25 tripartite motif containing 25 TRIM5 tripartite motif containing 5 UBE2L6 ubiquitin-conjugating enzyme E2L 6 USP18 ubiquitin specific peptidase 18 ACTB Actin, beta B2M Beta-2-microglobulin GAPDH Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK1 Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $495.00 |