IL6 Signaling

Interleukin 6 Interleukin-6 (IL-6) is cytokine involved in the regulation of the immune and inflammatory response. IL6 also contributes to other processes including hematopoiesis, metabolism, and organ development. Disruption or attenuation of IL-6 signaling may contribute to a autoimmune and inflammatory diseases, metabolic abnormalities, osteoporosis and malignancies. After interaction with the IL6 receptor, IL6 exerts its effects through a signaling cascade leading to the activation of the JAK/STAT and MAPK pathways. The result is transcriptional activation of a number or genes. The IL6 signaling primer library contains 88 primer sets involved in key aspects of these cellular processes and enables one to gain insight into the role of this pathway in a particular cellular context.

A ready-to-use Human IL-6 Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against a selected group of interleukin-6 signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

A2M alpha-2-macroglobulin

ABCB1 ATP-binding cassette, sub-family B (MDR/TAP), member 1

AGT angiotensinogen (serpin peptidase inhibitor, clade A, member 8)

BAD BCL2-associated agonist of cell death

BCL2L1 BCL2-like 1

BCL6 B-cell CLL/lymphoma 6

CCND1 In multiple Geneids

CD44 CD44 molecule (Indian blood group)

CEBPB CCAAT/enhancer binding protein (C/EBP), beta

CLCF1 cardiotrophin-like cytokine factor 1

CNTF ciliary neurotrophic factor

CNTFR ciliary neurotrophic factor receptor

CREB1 cAMP responsive element binding protein 1

CREBBP CREB binding protein

CRLF1 cytokine receptor-like factor 1

CRP C-reactive protein, pentraxin-related

CTF1 cardiotrophin 1

CYP19A1 cytochrome P450, family 19, subfamily A, polypeptide 1

EGFR epidermal growth factor receptor

ELK1 ELK1, member of ETS oncogene family

EP300 E1A binding protein p300

GAB1 GRB2-associated binding protein 1

GAS6 growth arrest-specific 6

GATA1 GATA binding protein 1 (globin transcription factor 1)

GRB2 growth factor receptor-bound protein 2

GSK3B glycogen synthase kinase 3 beta

HBEGF heparin-binding EGF-like growth factor

HCK HCK proto-oncogene, Src family tyrosine kinase

HDAC1 histone deacetylase 1

HRAS Harvey rat sarcoma viral oncogene homolog

IL11 interleukin 11

IL11RA interleukin 11 receptor, alpha

IL31 interleukin 31

IL31RA interleukin 13 receptor, alpha

IL4 interleukin 4

IL6 interleukin 6

IL6R interleukin 6 receptor

IL6ST interleukin 6 signal transducer

IL8 interleukin 8

IRF1 interferon regulatory factor 1

JAK1 Janus kinase 1

JAK2 Janus kinase 2

JUN jun proto-oncogene

JUNB jun B proto-oncogene

JUNB jun B proto-oncogene

LIFR leukemia inhibitory factor receptor alpha

MAP2K1 mitogen-activated protein kinase kinase 1

MAP2K2 mitogen-activated protein kinase kinase 2

MAP2K4 mitogen-activated protein kinase kinase 4

MAP3K7 mitogen-activated protein kinase kinase kinase 7

MAPK1 mitogen-activated protein kinase 1

MAPK3 mitogen-activated protein kinase 3

MCL1 myeloid cell leukemia 1

MYC MYCProto-Oncogene, BHLH Transcription Factor

NCOA1 nuclear receptor coactivator 1

NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

NLK nemo-like kinase

SPP1 secreted Phosphoprotein 1

OSM oncostatin M

OSMR oncostatin M receptor

PDGFA platelet-derived growth factor alpha polypeptide

PDK1 pyruvate dehydrogenase kinase, isozyme 1

PIAS3 protein inhibitor of activated STAT, 3

PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

PIK3R2 phosphoinositide-3-kinase, regulatory subunit 2 (beta)

PRDM1 PR/SET Domain 1

PRKCD protein kinase C, delta

PTPN11 protein tyrosine phosphatase, non-receptor type 11

RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

RAF1 Raf-1 proto-oncogene, serine/threonine kinase

REL v-rel avian reticuloendotheliosis viral oncogene homolog

RELA v-rel avian reticuloendotheliosis viral oncogene homolog A

RPS6KB1 ribosomal protein S6 kinase, 70kDa, polypeptide 1

SHC1 SHC (Src homology 2 domain containing) transforming protein 1

SOCS1 suppressor of cytokine signaling 1

SOCS3 suppressor of cytokine signaling 3

SOS1 son of sevenless homolog 1 (Drosophila)

SRC SRC proto-oncogene, non-receptor tyrosine kinase

STAT1 signal transducer and activator of transcription 1, 91kDa

STAT3 signal transducer and activator of transcription 3 (acute-phase response factor)

TIMP1 TIMP metallopeptidase inhibitor 1

TLR4 toll-like receptor 4

TNFAIP6 TNF Alpha Induced Protein 6

TNF tumor necrosis factor

TRIM8 tripartite motif containing 8

TYK2 tyrosine kinase 2

VAV1 vav 1 guanine nucleotide exchange factor

VIP vasoactive intestinal peptide

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPD Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a
$495.00
HIL6-I
A ready-to-use Mouse IL-6 Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against a selected group of interleukin-6 signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

A2M alpha-2-macroglobulin

Abcb1 ATP-binding cassette, sub-family B (MDR/TAP), member 1

Agt angiotensinogen (serpin peptidase inhibitor, clade A, member 8)

Bad BCL2-associated agonist of cell death

Bcl2L1 BCL2-like 1

Bcl6 B-cell CLL/lymphoma 6

Ccnd1 In multiple Geneids

Cd44 CD44 molecule (Indian blood group)

Cebpb CCAAT/enhancer binding protein (C/EBP), beta

Clcf1 cardiotrophin-like cytokine factor 1

Cntf ciliary neurotrophic factor

Cntfr ciliary neurotrophic factor receptor

Creb1 cAMP responsive element binding protein 1

Crebbp CREB binding protein

Crlf1 cytokine receptor-like factor 1

Crp C-reactive protein, pentraxin-related

Ctf1 cardiotrophin 1

Cyp19A1 cytochrome P450, family 19, subfamily A, polypeptide 1

Egfr epidermal growth factor receptor

Elk1 ELK1, member of ETS oncogene family

Ep300 E1A binding protein p300

Gab1 GRB2-associated binding protein 1

Gas6 growth arrest-specific 6

Gata1 GATA binding protein 1 (globin transcription factor 1)

Grb2 growth factor receptor-bound protein 2

Gsk3B glycogen synthase kinase 3 beta

Hbegf heparin-binding EGF-like growth factor

Hck HCK proto-oncogene, Src family tyrosine kinase

Hdac1 histone deacetylase 1

Hras Harvey rat sarcoma viral oncogene homolog

Il11 interleukin 11

Il11Ra interleukin 11 receptor, alpha

Il31 interleukin 31

Il31Ra interleukin 13 receptor, alpha

Il4 interleukin 4

Il6 interleukin 6

Il6R interleukin 6 receptor

Il6St interleukin 6 signal transducer

Il8 interleukin 8

Irf1 interferon regulatory factor 1

Jak1 Janus kinase 1

Jak2 Janus kinase 2

Jun jun proto-oncogene

Junb jun B proto-oncogene

Junb jun B proto-oncogene

Lifr leukemia inhibitory factor receptor alpha

Map2K1 mitogen-activated protein kinase kinase 1

Map2K2 mitogen-activated protein kinase kinase 2

Map2K4 mitogen-activated protein kinase kinase 4

Map3K7 mitogen-activated protein kinase kinase kinase 7

Mapk1 mitogen-activated protein kinase 1

Mapk3 mitogen-activated protein kinase 3

Mcl1 myeloid cell leukemia 1

Myc MYCProto-Oncogene, BHLH Transcription Factor

Ncoa1 nuclear receptor coactivator 1

Nfkb1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Nlk nemo-like kinase

Spp1 secreted Phosphoprotein 1

Osm oncostatin M

Osmr oncostatin M receptor

Pdgfa platelet-derived growth factor alpha polypeptide

Pdk1 pyruvate dehydrogenase kinase, isozyme 1

Pias3 protein inhibitor of activated STAT, 3

Pik3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Pik3R2 phosphoinositide-3-kinase, regulatory subunit 2 (beta)

Prdm1 PR/SET Domain 1

Prkcd protein kinase C, delta

Ptpn11 protein tyrosine phosphatase, non-receptor type 11

Rac1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

Raf1 Raf-1 proto-oncogene, serine/threonine kinase

Rel v-rel avian reticuloendotheliosis viral oncogene homolog

Rela v-rel avian reticuloendotheliosis viral oncogene homolog A

Rps6Kb1 ribosomal protein S6 kinase, 70kDa, polypeptide 1

Shc1 SHC (Src homology 2 domain containing) transforming protein 1

Socs1 suppressor of cytokine signaling 1

Socs3 suppressor of cytokine signaling 3

Sos1 son of sevenless homolog 1 (Drosophila)

Src SRC proto-oncogene, non-receptor tyrosine kinase

Stat1 signal transducer and activator of transcription 1, 91kDa

Stat3 signal transducer and activator of transcription 3 (acute-phase response factor)

Timp1 TIMP metallopeptidase inhibitor 1

Tlr4 toll-like receptor 4

Tnfaip6 TNF Alpha Induced Protein 6

Tnf tumor necrosis factor

Trim8 tripartite motif containing 8

Tyk2 tyrosine kinase 2

Vav1 vav 1 guanine nucleotide exchange factor

Vip vasoactive intestinal peptide

Actb Actin, beta

B2m Beta-2-microglobulin

Gapd Glyceraldehyde-3-phosphate dehydrogenase

Gusb Glucuronidase, beta

Hprt1 Hypoxanthine phosphoribosyltransferase 1

Pgk Phosphoglycerate kinase 1

Ppia Peptidylprolyl isomerase A

Rpl13a Ribosomal protein L13a
$495.00
MIL6-I