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Immunogenic Cell Death

Tumor cells have acquired mechanisms to resist anti-tumor immunity. The ability to evade the immune system is an important step for tumor establishment. As part of the tumor microenvironment (TME), normal cells are recruited to the tumor or altered by the tumor to form the tumor-associated stroma, which is important for tumor etiology and progression. Understanding the molecular signaling and extracellular interactions involved in this process allows for the development of novel therapies to overcome tumor immunity. Induction of immunogenic cell death (ICD) is a type of cell death eliciting an immune response and it is a relevant target to disrupt the immunosuppressive TME and promote immune surveillance. ICD may be induced by radiation, chemotherapeutics, and targeted therapies. ICD is associated with several processes in dying cells including calreticulin cell-surface translocation, extracellular release of damage-associated molecular patterns (DAMPs) such as HMGB1, heat shock proteins, and the production of Type I interferons. The Immunogenic Cell Death Primer Library contains 88 primer sets to amplify genes associated with ICD and DAMPs.

Human Immunogenic Cell Death Primer Library
A ready-to-use Human Immunogenic Cell Death PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against immunogenic cell death genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (40 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ACKR3 atypical chemokine receptor 3

AGER advanced glycosylation end product-specific receptor

ANXA1 annexin A1

APAF1 apoptotic peptidase activating factor 1

ATF4 activating transcription factor 4

ATF6 activating transcription factor 6

ATG5 autophagy related 5

ATG7 autophagy related 7

BAK1 BCL2-antagonist/killer 1

BAX BCL2-associated X protein

BIRC5 baculoviral IAP repeat containing 5

CALR calreticulin

CASP1 caspase 1, apoptosis-related cysteine peptidase

CASP8 caspase 8, apoptosis-related cysteine peptidase

CCL2 chemokine (C-C motif) ligand 2

CCL3 chemokine (C-C motif) ligand 3

CD276 CD276 molecule

CD40 CD40 molecule, TNF receptor superfamily member 5

CD47 CD47 molecule

CD80 CD80 molecule

CD86 CD86 molecule

CTLA4 cytotoxic T-lymphocyte-associated protein 4

CXCL1 chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)

CXCL10 chemokine (C-X-C motif) ligand 10

CXCL12 chemokine (C-X-C motif) ligand 12

CXCL15 chemokine (C-X-C motif) ligand 15

CXCL9 chemokine (C-X-C motif) ligand 9

CXCR4 chemokine (C-X-C motif) receptor 4

DDX58 DExD/H-Box Helicase 58

DEFB1 defensin, beta 1

DEFB103A defensin, beta 103A

EIF2AK2 eukaryotic translation initiation factor 2-alpha kinase 2

EIF2AK3 eukaryotic translation initiation factor 2-alpha kinase 3

EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa

ENTPD1 ectonucleoside triphosphate diphosphohydrolase 1

FPR1 formyl peptide receptor 1

HMGB1 high mobility group box 1

HSP90AA1 heat shock protein 90kDa alpha (cytosolic), class A member 1

HSP90AB1 heat shock protein 90kDa alpha (cytosolic), class B member 1

HSPA1A heat shock 70kDa protein 1A

HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

ICAM1 intercellular adhesion molecule 1

IFIH1 Interferon Induced With Helicase C Domain 1

IFNB1 interferon, beta 1, fibroblast

IFNG interferon, gamma

IL10 interleukin 10

IL12A interleukin 12A

IL12B interleukin 12B

IL17A interleukin 17A

IL18 interleukin 18

IL1A interleukin 1, alpha

IL1B interleukin 1, beta

IL1R1 interleukin 1 receptor, type I

IL23A interleukin 23, alpha subunit p19

IL2RA interleukin 2 receptor, alpha

IL6 interleukin 6

ITGAE integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)

KPNA2 karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

LAG3 lymphocyte-activation gene 3

LAMP1 lysosomal-associated membrane protein 1

LRP1 low density lipoprotein receptor-related protein 1

MAP1LC3B microtubule-associated protein 1 light chain 3 beta

MGP matrix Gla protein

MYD88 myeloid differentiation primary response 88

NFKB1 nuclear Factor Kappa B Subunit 1

NLRP3 NLR family, pyrin domain containing 3

P2RX7 purinergic receptor P2X, ligand gated ion channel, 7

P2RY2 purinergic receptor P2Y, G-protein coupled, 2

PANX1 pannexin 1

PDCD1 programmed cell death 1

PDIA3 protein disulfide isomerase family A, member 3

PTGS2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)

ROCK1 Rho-associated, coiled-coil containing protein kinase 1

S100A12 S100 calcium binding protein A12

S100A8 S100 calcium binding protein A8

S100A9 S100 calcium binding protein A9

SAP130 Sin3A-associated protein, 130kDa

SNAP25 synaptosomal-associated protein, 25kDa

SQSTM1 sequestosome 1

STING1 Stimulator Of Interferon Response CGAMP Interactor 1

TGFB1 transforming growth factor, beta 1

TLR2 toll-like receptor 2

TLR4 toll-like receptor 4

TLR9 toll-like receptor 9

TNF tumor necrosis factor

TNFSF10 tumor necrosis factor (ligand) superfamily, member 10

VAMP1 vesicle-associated membrane protein 1 (synaptobrevin 1)

XBP1 X-box binding protein 1

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPDH Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a
$495.00
HICD-I
Mouse Immunogenic Cell Death Primer Library
A ready-to-use Mouse Immunogenic Cell Death PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against immunogenic cell death genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (40 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ACKR3 atypical chemokine receptor 3

AGER advanced glycosylation end product-specific receptor

ANXA1 annexin A1

APAF1 apoptotic peptidase activating factor 1

ATF4 activating transcription factor 4

ATF6 activating transcription factor 6

ATG5 autophagy related 5

ATG7 autophagy related 7

BAK1 BCL2-antagonist/killer 1

BAX BCL2-associated X protein

BIRC5 baculoviral IAP repeat containing 5

CALR calreticulin

CASP1 caspase 1, apoptosis-related cysteine peptidase

CASP8 caspase 8, apoptosis-related cysteine peptidase

CCL2 chemokine (C-C motif) ligand 2

CCL3 chemokine (C-C motif) ligand 3

CD276 CD276 molecule

CD40 CD40 molecule, TNF receptor superfamily member 5

CD47 CD47 molecule

CD80 CD80 molecule

CD86 CD86 molecule

CTLA4 cytotoxic T-lymphocyte-associated protein 4

CXCL1 chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)

CXCL10 chemokine (C-X-C motif) ligand 10

CXCL12 chemokine (C-X-C motif) ligand 12

CXCL15 chemokine (C-X-C motif) ligand 15

CXCL9 chemokine (C-X-C motif) ligand 9

CXCR4 chemokine (C-X-C motif) receptor 4

DDX58 DExD/H-Box Helicase 58

DEFB1 defensin, beta 1

DEFB103A defensin, beta 103A

EIF2AK2 eukaryotic translation initiation factor 2-alpha kinase 2

EIF2AK3 eukaryotic translation initiation factor 2-alpha kinase 3

EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa

ENTPD1 ectonucleoside triphosphate diphosphohydrolase 1

FPR1 formyl peptide receptor 1

HMGB1 high mobility group box 1

HSP90AA1 heat shock protein 90kDa alpha (cytosolic), class A member 1

HSP90AB1 heat shock protein 90kDa alpha (cytosolic), class B member 1

HSPA1A heat shock 70kDa protein 1A

HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

ICAM1 intercellular adhesion molecule 1

IFIH1 Interferon Induced With Helicase C Domain 1

IFNB1 interferon, beta 1, fibroblast

IFNG interferon, gamma

IL10 interleukin 10

IL12A interleukin 12A

IL12B interleukin 12B

IL17A interleukin 17A

IL18 interleukin 18

IL1A interleukin 1, alpha

IL1B interleukin 1, beta

IL1R1 interleukin 1 receptor, type I

IL23A interleukin 23, alpha subunit p19

IL2RA interleukin 2 receptor, alpha

IL6 interleukin 6

ITGAE integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)

KPNA2 karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

LAG3 lymphocyte-activation gene 3

LAMP1 lysosomal-associated membrane protein 1

LRP1 low density lipoprotein receptor-related protein 1

MAP1LC3B microtubule-associated protein 1 light chain 3 beta

MGP matrix Gla protein

MYD88 myeloid differentiation primary response 88

NFKB1 nuclear Factor Kappa B Subunit 1

NLRP3 NLR family, pyrin domain containing 3

P2RX7 purinergic receptor P2X, ligand gated ion channel, 7

P2RY2 purinergic receptor P2Y, G-protein coupled, 2

PANX1 pannexin 1

PDCD1 programmed cell death 1

PDIA3 protein disulfide isomerase family A, member 3

PTGS2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)

ROCK1 Rho-associated, coiled-coil containing protein kinase 1

S100A12 S100 calcium binding protein A12

S100A8 S100 calcium binding protein A8

S100A9 S100 calcium binding protein A9

SAP130 Sin3A-associated protein, 130kDa

SNAP25 synaptosomal-associated protein, 25kDa

SQSTM1 sequestosome 1

STING1 Stimulator Of Interferon Response CGAMP Interactor 1

TGFB1 transforming growth factor, beta 1

TLR2 toll-like receptor 2

TLR4 toll-like receptor 4

TLR9 toll-like receptor 9

TNF tumor necrosis factor

TNFSF10 tumor necrosis factor (ligand) superfamily, member 10

VAMP1 vesicle-associated membrane protein 1 (synaptobrevin 1)

XBP1 X-box binding protein 1

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPDH Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a
$495.00
MICD-I