Human Pan Cell Death Primer Library

Contains 88 primer sets directed against cell death genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

Apoptosis-Intrinsic

BAD BCL2-associated agonist of cell death

BAK1 BCL2-antagonist/killer 1

BAX BCL2-associated X protein

BBC3 BCL2 binding component 3

BCL2 B-cell CLL/lymphoma 2

BCL2L1 BCL2-like 1

BID BH3 interacting domain death agonist

BIK BCL2-interacting killer (apoptosis-inducing)

BCL2L11 BCL2-like 11

CASP9 caspase 9, apoptosis-related cysteine peptidase

HRK harakiri, BCL2 interacting protein

MCL1 myeloid cell leukemia 1

Apoptosis-Extrinsic

CASP8 caspase 8, apoptosis-related cysteine peptidase

FAS Fas cell surface death receptor

FASLG Fas ligand (TNF superfamily, member 6)

TNF tumor necrosis factor

TNFRSF10A tumor necrosis factor receptor superfamily, member 10a

TNFRSF10B tumor necrosis factor receptor superfamily, member 10b

TNFRSF10C tumor necrosis factor receptor superfamily, member 10c, decoy without an intracellular domain

TNFRSF1A tumor necrosis factor receptor superfamily, member 1A

TNFRSF1B tumor necrosis factor receptor superfamily, member 1B

TNFRSF25 tumor necrosis factor receptor superfamily, member 25

TNFSF10 tumor necrosis factor (ligand) superfamily, member 10

TNFSF12 tumor necrosis factor (ligand) superfamily, member 12

Autophagy

ATG12 autophagy related 12

ATG14 autophagy related 14

ATG16L1 autophagy related 16-like 1 (S. cerevisiae)

ATG5 autophagy related 5

ATG7 autophagy related 7

BECN1 beclin 1, autophagy related

GABARAP GABA(A) receptor-associated protein

MAP1LC3A microtubule Associated Protein 1 Light Chain 3 Alpha

SQSTM1 sequestosome 1

TFEB transcription factor EB

ULK1 unc-51 like autophagy activating kinase 1

ULK2 unc-51 like autophagy activating kinase 2

Ferroptosis

ACSF2 acyl-CoA synthetase family member 2

ATP5G3 ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C3 (subunit 9)

CS citrate synthase

GCLC glutamate-cysteine ligase, catalytic subunit

GLS2 glutaminase 2 (liver, mitochondrial)

GOT1 glutamic-oxaloacetic transaminase 1, soluble

GPX4 glutathione peroxidase 4

IREB2 iron-responsive element binding protein 2

RPL8 ribosomal protein L8

SLC1A5 solute carrier family 1 (neutral amino acid transporter), member 5

SLC7A11 solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11

TFRC transferrin receptor

Necroptosis

BIRC2 baculoviral IAP repeat containing 2

BIRC3 baculoviral IAP repeat containing 3

CFLAR CASP8 and FADD-like apoptosis regulator

CYLD cylindromatosis (turban tumor syndrome)

FADD Fas (TNFRSF6)-associated via death domain

MLKL mixed lineage kinase domain-like

RIPK1 receptor (TNFRSF)-interacting serine-threonine kinase 1

RIPK3 receptor-interacting serine-threonine kinase 3

TLR3 toll-like receptor 3

TLR4 toll-like receptor 4

TRADD TNFRSF1A-associated via death domain

TRAF2 TNF receptor-associated factor 2

Pyroptosis

AIM2 absent in melanoma 2

CASP1 caspase 1, apoptosis-related cysteine peptidase

LAMP2 lysosomal-associated membrane protein 2

CASP4 caspase 4, apoptosis-related cysteine peptidase

CASP5 caspase 5, apoptosis-related cysteine peptidase

GSDMD gasdermin D

IL18 interleukin 18

IL1B interleukin 1, beta

NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

NLRC4 NLR family, CARD domain containing 4

NLRP1 NLR family, pyrin domain containing 1

NLRP3 NLR family, pyrin domain containing 3

NETosis

ELANE elastase, neutrophil expressed

CTSG cathepsin G

AZU1 azurocidin 1

MPO myeloperoxidase

PADI4 peptidyl Arginine Deiminase 4

Entosis

CDH1 cadherin 1, type 1, E-cadherin (epithelial)

CDH3 cadherin 3, type 1, P-cadherin (placental)

RHOA ras homolog family member A

Methuosis

HRAS Harvey rat sarcoma viral oncogene homolog

LAMP1 lysosomal-associated membrane protein 1

RAB5A RAB5A, Member RAS Oncogene Family

RAB7A RAB7A, Member RAS Oncogene Family

Parthanatos

AIF allograft inflammatory factor 1

MIF macrophage Migration Inhibitory Factor

PAR PAR protein

PARG poly (ADP-ribose) glycohydrolase

Housekeeping Genes

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPD Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a