Human NK Cell Mediated Toxicity Primer Library

Contains 88 primer sets directed against NK cell mediated toxicity genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

BID BH3 interacting domain death agonist

CASP3 caspase 3, apoptosis-related cysteine peptidase

CD244 CD244 molecule, natural killer cell receptor 2B4

CD247 CD247 molecule

CD48 CD48 molecule

CSF2 colony stimulating factor 2 (granulocyte-macrophage)

FAS Fas cell surface death receptor

FASLG Fas ligand (TNF superfamily, member 6)

FCER1G Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide

FCGR3A Fc fragment of IgG, low affinity IIIa, receptor (CD16a)

FCGR3B Fc fragment of IgG, low affinity IIIb, receptor (CD16b)

FYN FYN proto-oncogene, Src family tyrosine kinase

GRB2 growth factor receptor-bound protein 2

GZMB granzyme B

HCST hematopoietic cell signal transducer

HLA-A major histocompatibility complex, class I, A

HLA-B major histocompatibility complex, class I, B

HLA-C major histocompatibility complex, class I, C

HLA-E major histocompatibility complex, class I, E

HLA-G major histocompatibility complex, class I, G

ICAM1 intercellular adhesion molecule 1

ICAM2 intercellular adhesion molecule 2

IFNAR1 interferon (alpha, beta and omega) receptor 1

IFNAR2 interferon (alpha, beta and omega) receptor 2

IFNG interferon, gamma

IFNGR1 interferon gamma receptor 1

IFNGR2 interferon gamma receptor 2

ITGAL integrin, alpha L

ITGB2 integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)

KIR2DL1 killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1

KIR2DL2 killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 2

KIR2DL3 killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3

KIR2DL4 killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4

KIR2DL5A killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 5A

KIR2DS1 killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 1

KIR2DS2 killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 2

KIR2DS3 killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 3

KIR2DS4 killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 4

KIR2DS5 killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 5

KIR3DL1 killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1

KIR3DL2 killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 2

KLRC1 killer cell lectin-like receptor subfamily C, member 1

KLRD1 killer cell lectin-like receptor subfamily D, member 1 LAT linker for activation of T cells

LCK LCK proto-oncogene, Src family tyrosine kinase

LCP2 lymphocyte cytosolic protein 2

MAPK1 mitogen-activated protein kinase 1

MAPK3 mitogen-activated protein kinase 3

MICA MHC class I polypeptide-related sequence A

MICB MHC class I polypeptide-related sequence B

NCR1 natural cytotoxicity triggering receptor 1

NCR2 natural cytotoxicity triggering receptor 2

NCR3 natural cytotoxicity triggering receptor 3

NFATC1 nuclear factor of activated T-cells, cytoplasmic, calcineurin- dependent 1

NFATC2 nuclear factor of activated T-cells, cytoplasmic, calcineurin- dependent 2

PAK1 p21 protein (Cdc42/Rac)-activated kinase 1

PIK3CA phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha

PIK3CB phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta

PIK3CD phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit delta

PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

PIK3R2 phosphoinositide-3-kinase, regulatory subunit 2 (beta)

PIK3R3 phosphoinositide-3-kinase, regulatory subunit 3 (gamma)

PLCG1 phospholipase C, gamma 1

PLCG2 phospholipase C, gamma 2 (phosphatidylinositol-specific)

PRF1 perforin 1 (pore forming protein)

PTK2B protein Tyrosine Kinase 2 Beta

PTPN11 protein tyrosine phosphatase, non-receptor type 11

PTPN6 protein tyrosine phosphatase, non-receptor type 6

RAC1 ras-related C3 botulinum toxin substrate 1

RAC2 ras-related C3 botulinum toxin substrate 2

RAC3 ras-related C3 botulinum toxin substrate 3

RAF1 Raf-1 proto-oncogene, serine/threonine kinase

SH2D1A SH2 domain containing 1A

SH3BP2 SH3-domain binding protein 2

SHC1 SHC (Src homology 2 domain containing) transforming protein 1

SHC2 SHC (Src homology 2 domain containing) transforming protein 2

SYK spleen tyrosine kinase

TNF tumor necrosis factor

TNFRSF10B tumor necrosis factor receptor superfamily, member 10b

TNFSF10 tumor necrosis factor (ligand) superfamily, member 10

TYROBP TYRO protein tyrosine kinase binding protein

ULBP1 UL16 binding protein 1

ULBP2 UL16 binding protein 2

ULBP3 UL16 binding protein 3

VAV1 vav 1 guanine nucleotide exchange factor

VAV2 vav 2 guanine nucleotide exchange factor

VAV3 vav 3 guanine nucleotide exchange factor

ZAP70 zeta-chain (TCR) associated protein kinase 70kDa

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPDH Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a