Human G-Protein Coupled Receptor Signaling Primer Library

Contains 88 primer sets directed against amino acid metabolism genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



Gene List:

ADCY1 adenylate cyclase 1

ADCY2 adenylate cyclase 2

ADCY3 adenylate cyclase 3

AKT1 v-akt murine thymoma viral oncogene homolog 1

ARHGEF1 Rho guanine nucleotide exchange factor (GEF) 1

ARHGEF2 Rho/Rac guanine nucleotide exchange factor (GEF) 2

BRAF v-raf murine sarcoma viral oncogene homolog B1

CALM1 calmodulin 1 (phosphorylase kinase, delta)

CALM3 calmodulin 3 (phosphorylase kinase, delta)

CCND1 cyclin D2

CDC42 cell division cycle 42 (GTP binding protein, 25kDa)

CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1)

CDKN1B cyclin-dependent kinase inhibitor 1B (p27, Kip1)

CHUK conserved helix-loop-helix ubiquitous kinase

CREB1 cAMP responsive element binding protein 1

CRK v-crk sarcoma virus CT10 oncogene homolog (avian)

EGFR epidermal growth factor receptor

ELK1 ELK1, member of ETS oncogene family

ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

FOS FBJ murine osteosarcoma viral oncogene homolog

GNA12 guanine nucleotide binding protein (G protein) alpha 12

GNA13 guanine nucleotide binding protein (G protein), alpha 13

GNAI1 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1

GNAI2 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2

GNAQ guanine nucleotide binding protein (G protein), q polypeptide

GNAS GNAS complex locus

GNB3 guanine nucleotide binding protein (G protein), beta polypeptide 3

GNG2 guanine nucleotide binding protein (G protein), gamma 2

GRB2 growth factor receptor-bound protein 2

HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog

IKBKB inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta

IKBKG inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma

ITPR1 inositol 1,4,5-trisphosphate receptor, type 1

JUN jun proto-oncogene

KDR kinase insert domain receptor (a type III receptor tyrosine kinase)

KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

MAP2K1 mitogen-activated protein kinase kinase 1

MAP2K2 mitogen-activated protein kinase kinase 2

MAP2K4 mitogen-activated protein kinase kinase 4

MAP3K1 mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase

MAP3K5 mitogen-activated protein kinase kinase kinase 5

MAPK1 mitogen-activated protein kinase 1

MAPK10 mitogen-activated protein kinase 10

MAPK14 mitogen-activated protein kinase 14

MAPK3 mitogen-activated protein kinase 3

MAPK7 mitogen-activated protein kinase 7

MAPK8 mitogen-activated protein kinase 8

MAPK9 mitogen-activated protein kinase 9

NFATC1 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1

NFATC2 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2

NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

NFKB2 nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)

NFKBIA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

NRAS neuroblastoma RAS viral (v-ras) oncogene homolog

PAK1 p21 protein (Cdc42/Rac)-activated kinase 1

PAK2 p21 protein (Cdc42/Rac)-activated kinase 2

PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide

PIK3CB phosphoinositide-3-kinase, catalytic, beta polypeptide

PIK3CD phosphoinositide-3-kinase, catalytic, delta polypeptide

PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

PIK3R2 phosphoinositide-3-kinase, regulatory subunit 2 (beta)

PLCB1 phospholipase C, beta 1 (phosphoinositide-specific)

PPP3CA protein phosphatase 3, catalytic subunit, alpha isozyme

PRKACA protein kinase, cAMP-dependent, catalytic, alpha

PRKCA protein kinase C, alpha

PRKCB protein kinase C, beta

PRKCD protein kinase C, delta

PRKCE protein kinase C, epsilon

PRKCZ protein kinase C, zeta

PTK2 PTK2 protein tyrosine kinase 2

PTK2B protein tyrosine kinase 2, beta

PXN paxillin

RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

RAC2 ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)

RAF1 v-raf-1 murine leukemia viral oncogene homolog 1

RAP1A RAP1A, member of RAS oncogene family

RELA v-rel reticuloendotheliosis viral oncogene homolog A (avian)

RELB v-rel reticuloendotheliosis viral oncogene homolog B

RHOA ras homolog family member A

RHOB ras homolog family member B

RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

RAC2 ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)

SHC1 SHC (Src homology 2 domain containing) transforming protein 1

SOS1 son of sevenless homolog 1 (Drosophila)

SRC v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

SRF serum response factor (c-fos serum response element- binding transcription factor)

STAT1 signal transducer and activator of transcription 1, 91kDa

STAT3 signal transducer and activator of transcription 3 (acute-phase response factor)

ACTB actin, beta

B2M beta-2-microglobulin

GAPDH glyceraldehyde-3-phosphate dehydrogenase

GUSB glucuronidase, beta

HPRT1 hypoxanthine phosphoribosyltransferase 1

PGK1 phosphoglycerate kinase 1

PPIA peptidylprolyl isomerase A (cyclophilin A)

RPL13A ribosomal protein L13a