Human EGFR Signaling Primer Library

Contains 88 primer sets directed against EGFR signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

AKT1 v-akt murine thymoma viral oncogene homolog 1

AKT2 v-akt murine thymoma viral oncogene homolog 2

AKT3 v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)

ARAF v-raf murine sarcoma 3611 viral oncogene homolog

ATF2 activating transcription factor 2

BCL2 B-cell CLL/lymphoma 2

BRAF v-raf murine sarcoma viral oncogene homolog B1

CAV1 caveolin 1, caveolae protein, 22kDa

CAV2 caveolin 2

CASP3 caspase 3, apoptosis-related cysteine peptidase

CASP9 caspase 9, apoptosis-related cysteine peptidase

CBL Cbl proto-oncogene, E3 ubiquitin protein ligase

CHUK conserved helix-loop-helix ubiquitous kinase

DUSP1 dual specificity phosphatase 1

DUSP6 dual specificity phosphatase 6

EGF epidermal growth factor

EGFR epidermal growth factor receptor

ELK1 ELK1, member of ETS oncogene family

EPS8 epidermal growth factor receptor pathway substrate 8

ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

FASLG Fas ligand (TNF superfamily, member 6)

FOS FBJ murine osteosarcoma viral oncogene homolog

GAB1 GRB2-associated binding protein 1

GRB2 growth factor receptor-bound protein 2

HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog

IKBKB inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta

IKBKE inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon

IKBKG inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma

JAK1 Janus kinase 1

JAK2 Janus kinase 2

JUN jun proto-oncogene

KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

MAP2 microtubule-associated protein 2

MAP2K1 mitogen-activated protein kinase kinase 1

MAP2K2 mitogen-activated protein kinase kinase 2

MAP2K3 mitogen-activated protein kinase kinase 3

MAP2K4 mitogen-activated protein kinase kinase 4

MAP2K5 mitogen-activated protein kinase kinase 5

MAP2K6 mitogen-activated protein kinase kinase 6

MAP2K7 mitogen-activated protein kinase kinase 7

MAP3K1 mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase

MAP3K2 mitogen-activated protein kinase kinase kinase 2

MAP3K3 mitogen-activated protein kinase kinase kinase 3

MAPK1 mitogen-activated protein kinase 1

MAPK10 mitogen-activated protein kinase 10

MAPK12 mitogen-activated protein kinase 12

MAPK14 mitogen-activated protein kinase 14

MAPK3 mitogen-activated protein kinase 3

MAPK8 mitogen-activated protein kinase 8

MAPK9 mitogen-activated protein kinase 9

MBP myelin basic protein

MRAS muscle RAS oncogene homolog

MYC V-myc myelocytomatosis viral oncogene homolog (avian)

NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

NRAS neuroblastoma RAS viral (v-ras) oncogene homolog

PAK1 p21 protein (Cdc42/Rac)-activated kinase 1

PDGFB platelet-derived growth factor beta polypeptide

PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide

PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

PIK3R2 phosphoinositide-3-kinase, regulatory subunit 2 (beta)

PLCB3 phospholipase C, beta 3 (phosphatidylinositol- specific)

PLCG1 phospholipase C, gamma 1

PRKCA protein kinase C, alpha

PRKCB protein kinase C, beta

PRKCD protein kinase C, delta

PRKCE protein kinase C, epsilon

PRKCG protein kinase C, gamma

PTEN phosphatase and tensin homolog

RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

RAC2 ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)

RAF1 v-raf-1 murine leukemia viral oncogene homolog 1

RAP1A RAP1A, member of RAS oncogene family

RAP1B RAP1B, member of RAS oncogene family

REL v-rel reticuloendotheliosis viral oncogene homolog (avian)

RELA v-rel reticuloendotheliosis viral oncogene homolog A (avian)

RELB v-rel reticuloendotheliosis viral oncogene homolog B

RHOA ras homolog family member A

RHOB ras homolog family member B

RHOC ras homolog family member C

RHOD ras homolog family member D

RHOG ras homolog family member G

RRAS related RAS viral (r-ras) oncogene homolog

SHC1 SHC (Src homology 2 domain containing) transforming protein 1

SIPA1 signal-induced proliferation-associated 1

SRC v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

STAT1 signal transducer and activator of transcription 1, 91kDa

STAT3 signal transducer and activator of transcription 3 (acute-phase response factor)

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPDH Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a