Estrogens regulate reproduction, bone density, the cardiovascular system and metabolism. Estrogen acts mainly through the nuclear receptors ESR1 (ER-alpha) and ESR2 (ER-beta), which bind estrogen response elements in target genes and recruit coactivators or corepressors to control transcription. Estrogen also signals rapidly through the membrane receptor GPER1 and through cross-talk with growth factor pathways such as MAPK and PI3K-AKT. Estrogen receptor signaling drives the growth of most breast cancers and is the target of endocrine therapies such as tamoxifen and aromatase inhibitors.
A ready-to-use Human Estrogen Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against estrogen signaling genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: ADCYAP1 adenylate cyclase activating polypeptide 1 (pituitary) BCAR1 breast cancer anti-estrogen resistance 1 BCAR3 breast cancer anti-estrogen resistance 3 CARM1 coactivator-associated arginine methyltransferase 1 CEBPB CCAAT/enhancer binding protein (C/EBP), beta CYP19A1 cytochrome P450, family 19, subfamily A, polypeptide 1 CYP1B1 cytochrome P450, family 1, subfamily B, polypeptide 1 DDX54 DEAD (Asp-Glu-Ala-Asp) box polypeptide 54 EBAG9 estrogen receptor binding site associated, antigen, 9 EGF epidermal growth factor ERG v-ets erythroblastosis virus E26 oncogene homolog (avian) ESR1 estrogen receptor 1 ESR2 estrogen receptor 2 (ER beta) ESRRA estrogen-related receptor alpha ESRRG estrogen-related receptor gamma FGF2 fibroblast growth factor 2 (basic) FOXO1 forkhead box O1 FSHB follicle stimulating hormone, beta polypeptide FSHR follicle stimulating hormone receptor GABARAPL1 GABA(A) receptor-associated protein like 1 GHRH growth hormone releasing hormone GNL3 guanine nucleotide binding protein-like 3 (nucleolar) GNRH1 gonadotropin-releasing hormone 1 (luteinizing- releasing hormone) HMGA1 high mobility group AT-hook 1 HMGB1 high mobility group box 1 HMGB2 high mobility group box 2 HSD17B2 hydroxysteroid (17-beta) dehydrogenase 2 HSD17B8 hydroxysteroid (17-beta) dehydrogenase 8 HSPB8 heat shock 22kDa protein 8 IGF1 insulin-like growth factor 1 (somatomedin C) IL1A interleukin 1, alpha IL1B interleukin 1, beta INHA inhibin, alpha ISG20 interferon stimulated exonuclease gene 20kDa LHCGR luteinizing hormone/choriogonadotropin receptor TSKU tsukushi small leucine rich proteoglycan homolog (Xenopus laevis) MKNK2 MAP kinase interacting serine/threonine kinase 2 MPG N-methylpurine-DNA glycosylase MTA1 metastasis associated 1 NCOA1 nuclear receptor coactivator 1 NCOA3 nuclear receptor coactivator 3 NCOA5 nuclear receptor coactivator 5 NCOA6 nuclear receptor coactivator 6 NCOA7 nuclear receptor coactivator 7 NFATC4 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 NR0B2 nuclear receptor subfamily 0, group B, member 2 NR1I3 nuclear receptor subfamily 1, group I, member 3 NR2C2 nuclear receptor subfamily 2, group C, member 2 NR6A1 nuclear receptor subfamily 6, group A, member 1 NRG1 neuregulin 1 NRIP1 nuclear receptor interacting protein 1 OVGP1 oviductal glycoprotein 1, 120kDa PELP1 proline, glutamate and leucine rich protein 1 PGR progesterone receptor PHB2 prohibitin 2 PLG plasminogen POU4F1 POU class 4 homeobox 1 PPARA peroxisome proliferator-activated receptor alpha PPARGC1A peroxisome proliferator-activated receptor gamma, coactivator 1 alpha PPARGC1B peroxisome proliferator-activated receptor gamma, coactivator 1 beta PPID peptidylprolyl isomerase D RERG RAS-like, estrogen-regulated, growth inhibitor RLN1 relaxin 1 SAFB scaffold attachment factor B SAFB2 scaffold attachment factor B2 SRD5A2 steroid-5-alpha-reductase, alpha polypeptide 2 (3- oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) SREBF1 sterol regulatory element binding transcription factor 1 STS In multiple Geneids SULT1E1 sulfotransferase family 1E, estrogen-preferring, member 1 TAF10 TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa TCF7 transcription factor 7 (T-cell specific, HMG-box) TFF1 trefoil factor 1 TNF tumor necrosis factor TRIM16 tripartite motif containing 16 TRIM25 tripartite motif containing 25 UGT1A8 UDP glucuronosyltransferase 1 family, polypeptide A8 UGT1A3 UDP glucuronosyltransferase 1 family, polypeptide A3 UGT1A4 UDP glucuronosyltransferase 1 family, polypeptide A4 UGT2A1 UDP glucuronosyltransferase 2 family, polypeptide A1, complex locus UGT2B15 UDP glucuronosyltransferase 2 family, polypeptide B15 UGT2B4 UDP glucuronosyltransferase 2 family, polypeptide B4 UGT2B7 UDP glucuronosyltransferase 2 family, polypeptide B7 NR0B1 nuclear receptor subfamily 0, group B, member 1 GNRH2 gonadotropin-releasing hormone 2 NRG3 neuregulin 3 NRG4 neuregulin 4 AR androgen receptor ACTB Actin, beta B2M Beta-2-microglobulin GAPDH Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK1 Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $595.00 |
A ready-to-use Mouse Estrogen Receptor Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against estrogen signaling genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: Adcyap1 adenylate cyclase activating polypeptide 1 (pituitary) Bcar1 breast cancer anti-estrogen resistance 1 Bcar3 breast cancer anti-estrogen resistance 3 Carm1 coactivator-associated arginine methyltransferase 1 Cebpb CCAAT/enhancer binding protein (C/EBP), beta Cyp19A1 cytochrome P450, family 19, subfamily A, polypeptide 1 Cyp1B1 cytochrome P450, family 1, subfamily B, polypeptide 1 Ddx54 DEAD (Asp-Glu-Ala-Asp) box polypeptide 54 Ebag9 estrogen receptor binding site associated, antigen, 9 Egf epidermal growth factor Erg v-ets erythroblastosis virus E26 oncogene homolog (avian) Esr1 estrogen receptor 1 Esr2 estrogen receptor 2 (ER beta) Esrra estrogen-related receptor alpha Esrrg estrogen-related receptor gamma Fgf2 fibroblast growth factor 2 (basic) Foxo1 forkhead box O1 Fshb follicle stimulating hormone, beta polypeptide Fshr follicle stimulating hormone receptor Gabarapl1 GABA(A) receptor-associated protein like 1 Ghrh growth hormone releasing hormone Gnl3 guanine nucleotide binding protein-like 3 (nucleolar) Gnrh1 gonadotropin-releasing hormone 1 (luteinizing-releasing hormone) Hmga1 high mobility group AT-hook 1 Hmgb1 high mobility group box 1 Hmgb2 high mobility group box 2 Hsd17B2 hydroxysteroid (17-beta) dehydrogenase 2 Hsd17B8 hydroxysteroid (17-beta) dehydrogenase 8 Hspb8 heat shock 22kDa protein 8 Igf1 insulin-like growth factor 1 (somatomedin C) Il1A interleukin 1, alpha Il1B interleukin 1, beta Inha inhibin, alpha Isg20 interferon stimulated exonuclease gene 20kDa Lhcgr luteinizing hormone/choriogonadotropin receptor Tsku tsukushi small leucine rich proteoglycan homolog (Xenopus laevis) Mknk2 MAP kinase interacting serine/threonine kinase 2 Mpg N-methylpurine-DNA glycosylase Mta1 metastasis associated 1 Ncoa1 nuclear receptor coactivator 1 Ncoa3 nuclear receptor coactivator 3 Ncoa5 nuclear receptor coactivator 5 Ncoa6 nuclear receptor coactivator 6 Ncoa7 nuclear receptor coactivator 7 Nfatc4 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 Nfkb1 nuclear factor of kappa light polypeptide gene enhancer in B- cells 1 Nr0B2 nuclear receptor subfamily 0, group B, member 2 Nr1I3 nuclear receptor subfamily 1, group I, member 3 Nr2C2 nuclear receptor subfamily 2, group C, member 2 Nr6A1 nuclear receptor subfamily 6, group A, member 1 Nrg1 neuregulin 1 Nrip1 nuclear receptor interacting protein 1 Ovgp1 oviductal glycoprotein 1, 120kDa Pelp1 proline, glutamate and leucine rich protein 1 Pgr progesterone receptor Phb2 prohibitin 2 Plg plasminogen Pou4F1 POU class 4 homeobox 1 Ppara peroxisome proliferator-activated receptor alpha Ppargc1A peroxisome proliferator-activated receptor gamma, coactivator 1 alpha Ppargc1B peroxisome proliferator-activated receptor gamma, coactivator 1 beta Ppid peptidylprolyl isomerase D Rerg RAS-like, estrogen-regulated, growth inhibitor Rln1 relaxin 1 Safb scaffold attachment factor B Safb2 scaffold attachment factor B2 Srd5A2 steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) Srebf1 sterol regulatory element binding transcription factor 1 Sts In multiple Geneids Sult1E1 sulfotransferase family 1E, estrogen-preferring, member 1 Taf10 TAF10 RNA polymerase II, TATA box binding protein (TBP)- associated factor, 30kDa Tcf7 transcription factor 7 (T-cell specific, HMG-box) Tff1 trefoil factor 1 Tnf tumor necrosis factor Trim16 tripartite motif containing 16 Trim25 tripartite motif containing 25 Ugt1A8 UDP glucuronosyltransferase 1 family, polypeptide A8 Ugt1A3 UDP glucuronosyltransferase 1 family, polypeptide A3 Ugt1A4 UDP glucuronosyltransferase 1 family, polypeptide A4 Ugt2A1 UDP glucuronosyltransferase 2 family, polypeptide A1, complex locus Ugt2B15 UDP glucuronosyltransferase 2 family, polypeptide B15 Ugt2B4 UDP glucuronosyltransferase 2 family, polypeptide B4 Ugt2B7 UDP glucuronosyltransferase 2 family, polypeptide B7 Nr0B1 nuclear receptor subfamily 0, group B, member 1 Gnrh2 gonadotropin-releasing hormone 2 Nrg3 neuregulin 3 Nrg4 neuregulin 4 Ar androgen receptor Actb Actin, beta B2M Beta-2-microglobulin Gapdh Glyceraldehyde-3-phosphate dehydrogenase Gusb Glucuronidase, beta Hprt1 Hypoxanthine phosphoribosyltransferase 1 Pgk1 Phosphoglycerate kinase 1 Ppia Peptidylprolyl isomerase A Rpl13A Ribosomal protein L13a $595.00 |