DNA repair pathways correct damage to the genome and are essential for maintaining genomic stability. Major pathways include base excision repair (BER) for small base lesions, nucleotide excision repair (NER) for bulky adducts and UV damage, mismatch repair (MMR) for replication errors, and homologous recombination (HR) and non-homologous end joining (NHEJ) for double-strand breaks. Key genes include OGG1, XPA, MLH1, MSH2, BRCA1, BRCA2, RAD51, XRCC4 and PARP1. Defects in DNA repair lead to cancer predisposition, and DNA repair genes are important targets for cancer therapy.
A ready-to-use Human DNA Repair I PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against DNA repair genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: UNG Uracil-Dna Glycosylase SMUG1 Single-Strand-Selective Monofunctional Uracil-Dna Glycosylase 1 MBD4 Methyl-Cpg Binding Domain Protein 4 TDG Thymine-Dna Glycosylase OGG1 8-Oxoguanine Dna Glycosylase MUTYH Muty Homolog (E. Coli) NTHL1 Nth Endonuclease Iii-Like 1 (E. Coli) MPG N-Methylpurine-Dna Glycosylase NEIL1 Nei Endonuclease Viii-Like 1 (E. Coli) NEIL2 Nei Endonuclease Viii-Like 2 (E. Coli) APEX1 Apex Nuclease 1 APEX2 Apex Nuclease 2 LIG3 Ligase Iii, Dna, Atp-Dependent XRCC1 X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 1 PNKP Polynucleotide Kinase 3'-Phosphatase PARP1 Poly (Adp-Ribose) Polymerase 1 PARP2 Poly (Adp-Ribose) Polymerase 2 MGMT O-6-Methylguanine-Dna Methyltransferase ALKBH2 Alkb, Alkylation Repair Homolog 2 (E. Coli) ALKBH3 Alkb, Alkylation Repair Homolog 3 (E. Coli) TDP1 Tyrosyl-Dna Phosphodiesterase 1 MSH2 Muts Homolog 2, Colon Cancer, Nonpolyposis Type 1 MSH3 Muts Homolog 3 (E. Coli) MSH6 Muts Homolog 6 (E. Coli) MSH4 Muts Homolog 4 (E. Coli) MSH5 Muts Homolog 5 (E. Coli) PMS1 Pms1 Postmeiotic Segregation Increased 1 MLH1 Mutl Homolog 1, Colon Cancer, Nonpolyposis Type 2 PMS2 Pms2 Postmeiotic Segregation Increased 2 MLH3 Mutl Homolog 3 (E. Coli) PMS2L3 Postmeiotic Segregation Increased 2-Like 3 PMS2L4 Postmeiotic Segregation Increased 2-Like 4 XPC Xeroderma Pigmentosum, Complementation Group C RAD23B Rad23 Homolog B CETN2 Centrin, Ef-Hand Protein, 2 RAD23A Rad23 Homolog A XPA Xeroderma Pigmentosum, Complementation Group A RPA1 Replication Protein A1, 70Kda RPA2 Replication Protein A2, 32Kda RPA3 Replication Protein A3, 14Kda ERCC3 Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 3 ERCC2 Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 2 GTF2H1 General Transcription Factor Iih, Polypeptide 1, 62Kda GTF2H2 General Transcription Factor Iih, Polypeptide 2, 44Kda GTF2H3 General Transcription Factor Iih, Polypeptide 3, 34Kda GTF2H4 General Transcription Factor Iih, Polypeptide 4, 52Kda GTF2H5 General Transcription Factor Iih, Polypeptide 5 CDK7 Cyclin-Dependent Kinase 7 MNAT1 Menage A Trois Homolog 1, Cyclin H Assembly Factor (Xenopus Laevis) ERCC5 Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 5 ERCC1 Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 1 ERCC4 Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 4 LIG1 Ligase I, Dna, Atp-Dependent ERCC8 Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 ERCC6 Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 6 XAB2 Xpa Binding Protein 2 DDB1 Damage-Specific Dna Binding Protein 1, 127Kda DDB2 Damage-Specific Dna Binding Protein 2, 48Kda MMS19 MMS19 nucleotide excision repair homolog RAD51 Rad51 Homolog (Reca Homolog, E. Coli) RAD51L1 Rad51-Like 1 (S. Cerevisiae) RAD51C Rad51 Homolog C (S. Cerevisiae) RAD51L3 Rad51-Like 3 (S. Cerevisiae) DMC1 Dmc1 Dosage Suppressor Of Mck1 Homolog XRCC2 X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 2 XRCC3 X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 3 RAD52 Rad52 Homolog (S. Cerevisiae) RAD54L Rad54-Like (S. Cerevisiae) RAD54B Rad54 Homolog B (S. Cerevisiae) BRCA1 Breast Cancer 1, Early Onset BRCA2 Breast Cancer 2, Early Onset SHFM1 Split hand/foot malformation (ectrodactyly) type 1 RAD50 Rad50 Homolog (S. Cerevisiae) MRE11A Mre11 Meiotic Recombination 11 Homolog A NBN Nibrin (NBS1) MUS81 Mus81 Endonuclease Homolog (S. Cerevisiae) EME1 Essential Meiotic Endonuclease 1 Homolog 1 XRCC6BP1 Xrcc6 Binding Protein 1 XRCC6 X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6 XRCC5 X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 5 PRKDC Protein Kinase, Dna-Activated, Catalytic Polypeptide LIG4 Ligase Iv, Dna, Atp-Dependent XRCC4 X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 4 DCLRE1C Dna Cross-Link Repair 1C NUDT1 Nudix (Nucleoside Diphosphate Linked Moiety X)- Type Motif 1 NUDT2 Nudix (Nucleoside Diphosphate Linked Moiety X)- Type Motif 2 DUT Deoxyuridine Triphosphatase RRM2B Ribonucleotide Reductase M2 B (Tp53 Inducible) ACTB Actin, beta B2M Beta-2-microglobulin GAPD Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $495.00 |
A ready-to-use Human DNA Repair II PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against DNA repair genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: POLB Polymerase (Dna Directed), Beta POLG Polymerase (Dna Directed), Gamma POLD1 Polymerase (Dna Directed), Delta 1, Catalytic Subunit 125Kda POLE Polymerase (Dna Directed), Epsilon PCNA Proliferating Cell Nuclear Antigen REV3L Rev3-Like, Catalytic Subunit Of Dna Polymerase Zeta (Yeast) MAD2L1 Mad2 Mitotic Arrest Deficient-Like 1 (Yeast) MAD2L2 Mad2 Mitotic Arrest Deficient-Like 2 (Yeast) REV1 REV1 homolog POLH Polymerase (Dna Directed), Eta POLI Polymerase (Dna Directed) Iota POLQ Polymerase (Dna Directed), Theta POLK Polymerase (Dna Directed) Kappa POLL Polymerase (Dna Directed), Lambda POLM Polymerase (Dna Directed), Mu POLN Polymerase (Dna Directed) Nu FEN1 Flap Structure-Specific Endonuclease 1 TREX1 Three Prime Repair Exonuclease 1 TREX2 Three Prime Repair Exonuclease 2 EXO1 Exonuclease 1 SPO11 Spo11 Meiotic Protein Covalently Bound To Dsb Homolog (S. Cerevisiae) FLJ35220 Hypothetical Protein Flj35220 UBE2A Ubiquitin-Conjugating Enzyme E2A (Rad6 Homolog) UBE2B Ubiquitin-Conjugating Enzyme E2B (Rad6 Homolog) RAD18 Rad18 Homolog (S. Cerevisiae) UBE2V2 Ubiquitin-Conjugating Enzyme E2 Variant 2 UBE2V1 Ubiquitin-Conjugating Enzyme E2 Variant 1 UBE2N Ubiquitin-Conjugating Enzyme E2N (Ubc13 Homolog, Yeast) H2AFX H2A Histone Family, Member X CHAF1A Chromatin Assembly Factor 1, Subunit A (P150) BLM Bloom Syndrome, Recq Helicase-Like WRN Werner Syndrome, Recq Helicase-Like RECQL4 Recq Protein-Like 4 ATM Ataxia Telangiectasia Mutated FANCA Fanconi Anemia, Complementation Group A FANCB Fanconi Anemia, Complementation Group B FANCC Fanconi Anemia, Complementation Group C FANCD2 Fanconi Anemia, Complementation Group D2 FANCE Fanconi Anemia, Complementation Group E FANCF Fanconi Anemia, Complementation Group F FANCG Fanconi Anemia, Complementation Group G FANCL Fanconi Anemia, Complementation Group L DCLRE1A Dna Cross-Link Repair 1A DCLRE1B Dna Cross-Link Repair 1B RPA4 Replication Protein A4, 30Kda APTX Aprataxin NEIL3 Nei Endonuclease Viii-Like 3 (E. Coli) RECQL Recq Protein-Like (Dna Helicase Q1-Like) RECQL5 Recq Protein-Like 5 HELQ Helicase, POLQ-like RDM1 RAD52 motif 1 ATR Ataxia Telangiectasia And Rad3 Related RAD1 Rad1 Homolog (S. Pombe) RAD9A Rad9 Homolog A (S. Pombe) HUS1 Hus1 Checkpoint Homolog (S. Pombe) RAD17 Rad17 Homolog (S. Pombe) CHEK1 Chk1 Checkpoint Homolog (S. Pombe) CHEK2 Chk2 Checkpoint Homolog (S. Pombe) TP53 Tumor Protein P53 TP73 Tumor Protein P73 CDK2 Cyclin-Dependent Kinase 2 CDK4 Cyclin-Dependent Kinase 4 CDK5 Cyclin-Dependent Kinase 5 CDK5R1 Cyclin-Dependent Kinase 5, Regulatory Subunit 1 (P35) CDK5RAP1 Cdk5 Regulatory Subunit Associated Protein 1 CDK6 Cyclin-Dependent Kinase 6 CDK8 Cyclin-Dependent Kinase 8 CDKN1A Cyclin-Dependent Kinase Inhibitor 1A (P21, Cip1) CDKN1B Cyclin-Dependent Kinase Inhibitor 1B (P27, Kip1) CDKN2A Cyclin-Dependent Kinase Inhibitor 2A (Melanoma, P16, Inhibits Cdk4) CDKN2B Cyclin-Dependent Kinase Inhibitor 2B (P15, Inhibits Cdk4) CDKN2C Cyclin-Dependent Kinase Inhibitor 2C (P18, Inhibits Cdk4) CDKN2D Cyclin-Dependent Kinase Inhibitor 2D (P19, Inhibits Cdk4) CDKN3 Cyclin-Dependent Kinase Inhibitor 3 CCNA1 Cyclin A1 CCNA2 Cyclin A2 CCNB1 Cyclin B1 CCNB2 Cyclin B2 CCNC Cyclin C CCND1 Cyclin D1 CCND2 Cyclin D2 CCNE1 Cyclin E1 CCNE2 Cyclin E2 CCNF Cyclin F CCNG1 Cyclin G1 CCNG2 Cyclin G2 CCNH Cyclin H CCNT1 Cyclin T1 ACTB Actin, beta B2M Beta-2-microglobulin GAPD Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $495.00 |
A ready-to-use Mouse DNA Repair I PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against DNA repair genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: UNG Uracil-Dna Glycosylase SMUG1 Single-Strand-Selective Monofunctional Uracil-Dna Glycosylase 1 MBD4 Methyl-Cpg Binding Domain Protein 4 TDG Thymine-Dna Glycosylase OGG1 8-Oxoguanine Dna Glycosylase MUTYH Muty Homolog (E. Coli) NTHL1 Nth Endonuclease Iii-Like 1 (E. Coli) MPG N-Methylpurine-Dna Glycosylase NEIL1 Nei Endonuclease Viii-Like 1 (E. Coli) NEIL2 Nei Endonuclease Viii-Like 2 (E. Coli) APEX1 Apex Nuclease 1 APEX2 Apex Nuclease 2 LIG3 Ligase Iii, Dna, Atp-Dependent XRCC1 X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 1 PNKP Polynucleotide Kinase 3'-Phosphatase PARP1 Poly (Adp-Ribose) Polymerase 1 PARP2 Poly (Adp-Ribose) Polymerase 2 MGMT O-6-Methylguanine-Dna Methyltransferase ALKBH2 Alkb, Alkylation Repair Homolog 2 (E. Coli) ALKBH3 Alkb, Alkylation Repair Homolog 3 (E. Coli) TDP1 Tyrosyl-Dna Phosphodiesterase 1 MSH2 Muts Homolog 2, Colon Cancer, Nonpolyposis Type 1 MSH3 Muts Homolog 3 (E. Coli) MSH6 Muts Homolog 6 (E. Coli) MSH4 Muts Homolog 4 (E. Coli) MSH5 Muts Homolog 5 (E. Coli) PMS1 Pms1 Postmeiotic Segregation Increased 1 MLH1 Mutl Homolog 1, Colon Cancer, Nonpolyposis Type 2 PMS2 Pms2 Postmeiotic Segregation Increased 2 MLH3 Mutl Homolog 3 (E. Coli) PMS2L3 Postmeiotic Segregation Increased 2-Like 3 PMS2L4 Postmeiotic Segregation Increased 2-Like 4 XPC Xeroderma Pigmentosum, Complementation Group C RAD23B Rad23 Homolog B CETN2 Centrin, Ef-Hand Protein, 2 RAD23A Rad23 Homolog A XPA Xeroderma Pigmentosum, Complementation Group A RPA1 Replication Protein A1, 70Kda RPA2 Replication Protein A2, 32Kda RPA3 Replication Protein A3, 14Kda ERCC3 Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 3 ERCC2 Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 2 GTF2H1 General Transcription Factor Iih, Polypeptide 1, 62Kda GTF2H2 General Transcription Factor Iih, Polypeptide 2, 44Kda GTF2H3 General Transcription Factor Iih, Polypeptide 3, 34Kda GTF2H4 General Transcription Factor Iih, Polypeptide 4, 52Kda GTF2H5 General Transcription Factor Iih, Polypeptide 5 CDK7 Cyclin-Dependent Kinase 7 MNAT1 Menage A Trois Homolog 1, Cyclin H Assembly Factor (Xenopus Laevis) ERCC5 Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 5 ERCC1 Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 1 ERCC4 Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 4 LIG1 Ligase I, Dna, Atp-Dependent ERCC8 Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 ERCC6 Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 6 XAB2 Xpa Binding Protein 2 DDB1 Damage-Specific Dna Binding Protein 1, 127Kda DDB2 Damage-Specific Dna Binding Protein 2, 48Kda MMS19 MMS19 nucleotide excision repair homolog RAD51 Rad51 Homolog (Reca Homolog, E. Coli) RAD51L1 Rad51-Like 1 (S. Cerevisiae) RAD51C Rad51 Homolog C (S. Cerevisiae) RAD51L3 Rad51-Like 3 (S. Cerevisiae) DMC1 Dmc1 Dosage Suppressor Of Mck1 Homolog XRCC2 X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 2 XRCC3 X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 3 RAD52 Rad52 Homolog (S. Cerevisiae) RAD54L Rad54-Like (S. Cerevisiae) RAD54B Rad54 Homolog B (S. Cerevisiae) BRCA1 Breast Cancer 1, Early Onset BRCA2 Breast Cancer 2, Early Onset SHFM1 Split hand/foot malformation (ectrodactyly) type 1 RAD50 Rad50 Homolog (S. Cerevisiae) MRE11A Mre11 Meiotic Recombination 11 Homolog A NBN Nibrin (NBS1) MUS81 Mus81 Endonuclease Homolog (S. Cerevisiae) EME1 Essential Meiotic Endonuclease 1 Homolog 1 XRCC6BP1 Xrcc6 Binding Protein 1 XRCC6 X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6 XRCC5 X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 5 PRKDC Protein Kinase, Dna-Activated, Catalytic Polypeptide LIG4 Ligase Iv, Dna, Atp-Dependent XRCC4 X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 4 DCLRE1C Dna Cross-Link Repair 1C NUDT1 Nudix (Nucleoside Diphosphate Linked Moiety X)- Type Motif 1 NUDT2 Nudix (Nucleoside Diphosphate Linked Moiety X)- Type Motif 2 DUT Deoxyuridine Triphosphatase RRM2B Ribonucleotide Reductase M2 B (Tp53 Inducible) ACTB Actin, beta B2M Beta-2-microglobulin GAPD Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $595.00 |