Covid-19 (SARS-2)

Human infection by coronaviruses has become a significant health problem. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters cells using its large viral Spike protein which is activated by serine protease TMPRSS2. Following binding to its receptor, angiotensin-converting enzyme 2 (ACE2), the virus enters the cell. ACE2 is down-regulated which results in further angiotensin 2 accumulation. Activation of the angiotensin pathway system causes lung injury in response to the virus. A variety of signaling events occur in the lung epithelial and immune cells following infections by Covid-19 and other viruses. These include chemokine and cytokine induced processes, signaling pathways and transcription factors. SARS-2 (Covid-19) triggers the hosts innate immune system through the type I interferon system resulting in the activation of ISRE and IFN-beta sensitive promoter elements. Understanding the immune response to viral infection and the cascade of events that leads to tissue damage and death is important to developing diagnostic methods and new treatments.

A ready-to-use Human SARS-2 Covid-19 Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against Covid-19 related genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ACE angiotensin I converting enzyme

ACE2 angiotensin I converting enzyme 2

AGR2 anterior gradient 2

AGTR1 angiotensin II receptor, type 1

AGTR2 angiotensin II receptor, type 2

ANG angiogenin, ribonuclease, RNase A family, 5

BDKRB1 bradykinin receptor B1

BDKRB2 bradykinin receptor B2

CCL2 chemokine (C-C motif) ligand 2

CCL20 chemokine (C-C motif) ligand 20

CCL3 chemokine (C-C motif) ligand 3

CCL7 chemokine (C-C motif) ligand 7

CFB complement factor B

CMA1 chymase 1, mast cell

CSF3 colony stimulating factor 3 (granulocyte)

CTSG cathepsin G

CXCL1 chemokine (C-X-C motif) ligand 1

CXCL10 chemokine (C-X-C motif) ligand 10

CXCL17 chemokine (C-X-C motif) ligand 17

CXCL2 chemokine (C-X-C motif) ligand 2

DDX58 DEAD (Asp-Glu-Ala-Asp) box polypeptide 58

IER3 immediate early response 3

IFIH1 interferon induced with helicase C domain 1

IFIT1 interferon Induced Protein With Tetratricopeptide Repeats 1

IFITM3 interferon induced transmembrane protein 3

IFNA1 interferon, alpha 1

IFNA10 interferon, alpha 10

IFNA13 interferon, alpha 13

IFNA14 interferon, alpha 14

IFNA16 interferon, alpha 16

IFNA17 interferon, alpha 17

IFNA2 interferon, alpha 2

IFNA21 interferon, alpha 21

IFNA4 interferon, alpha 4

IFNA5 interferon, alpha 5

IFNA6 interferon, alpha 6

IFNA7 interferon, alpha 7

IFNA8 interferon, alpha 8

IFNAR1 interferon (alpha, beta and omega) receptor 1

IFNAR2 interferon (alpha, beta and omega) receptor 2

IFNB1 interferon, beta 1, fibroblast

IKBKE inhibitor of kappa light polypeptide gene enhancer in B- cells, kinase epsilon

IL10 interleukin 10

IL1B interleukin 1, beta

IL1RN interleukin 1 receptor antagonist

IL2 interleukin 2

IL2RA interleukin 2 receptor, alpha

IL6 interleukin 6

IL7 interleukin 7

IL8 interleukin 8

IRF3 interferon regulatory factor 3

IRF9 interferon regulatory factor 9

ISG15 ISG15 ubiquitin-like modifier

ISG20 interferon stimulated exonuclease gene 20kDa

JAK1 Janus kinase 1

MAS1 MAS1 proto-oncogene, G protein-coupled receptor

MAVS mitochondrial antiviral signaling protein

MX1 MX dynamin-like GTPase 1

NFKBIA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

NR3C2 nuclear receptor subfamily 3, group C, member 2

NRP1 neuropilin 1

OAS1 2'-5'-oligoadenylate synthetase 1, 40/46kDa

PGR progesterone receptor

PLSCR1 phospholipid scramblase 1

RARRES1 retinoic acid receptor responder (tazarotene induced) 1

REN renin

S100A12 S100 calcium binding protein A12

S100A8 S100 calcium binding protein A8

S100A9 S100 calcium binding protein A9

SAT1 spermidine/spermine N1-acetyltransferase 1

SERPINB3 serpin peptidase inhibitor, clade B (ovalbumin), member 3

SLC6A19 solute carrier family 6 (neutral amino acid transporter), member 19

SOCS3 suppressor of cytokine signaling 3

STAT1 signal transducer and activator of transcription 1, 91kDa

STAT2 signal transducer and activator of transcription 2, 113kDa

TBK1 TANK-binding kinase 1

TICAM1 toll-like receptor adaptor molecule 1

TLR3 toll-like receptor 3

TLR7 toll-like receptor 7

TMCC1 transmembrane and coiled-coil domain family 1

TMPRSS2 transmembrane protease, serine 2

TMPRSS4 transmembrane protease, serine 4

TNF tumor necrosis factor

TNFAIP3 tumor necrosis factor, alpha-induced protein 3

TOP2A topoisomerase (DNA) II alpha 170kDa

TRAF3 TNF receptor-associated factor 3

TYK2 tyrosine kinase 2

VEGFA vascular endothelial growth factor A

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPD Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a
$595.00
HSARS-I