Cyclic AMP (cAMP) is a second messenger that relays signals from many hormones and neurotransmitters. Activation of G protein coupled receptors coupled to Gs stimulates adenylyl cyclases to produce cAMP, while Gi coupled receptors inhibit its production and phosphodiesterases (PDEs) degrade it. cAMP activates protein kinase A (PKA), EPAC exchange factors and cyclic nucleotide gated ion channels. PKA phosphorylates many targets, including the transcription factor CREB, to regulate metabolism, gene transcription, memory, cardiac function and immune responses.
A ready-to-use Human cAMP Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against cyclic AMP signaling genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: ADCY1 adenylate cyclase 1 (brain) ADCY2 In multiple Geneids ADCY3 adenylate cyclase 3 ADCY4 In multiple Geneids ADCY5 adenylate cyclase 5 ADCY6 adenylate cyclase 6 ADCY7 adenylate cyclase 7 ADCY8 adenylate cyclase 8 (brain) ADCY9 adenylate cyclase 9 ADCY10 adenylate cyclase 10 (soluble) ADRA1A adrenoceptor alpha 1A ADRA2A adrenoceptor alpha 2A ADRB1 adrenoceptor beta 1 ADRB2 adrenoceptor beta 2, surface ADRB3 adrenoceptor beta 3 AKAP1 A kinase (PRKA) anchor protein 1 AKAP10 A kinase (PRKA) anchor protein 10 AKAP12 A kinase (PRKA) anchor protein 12 AKAP13 A kinase (PRKA) anchor protein 13 AKAP5 A kinase (PRKA) anchor protein 5 AKAP8 A kinase (PRKA) anchor protein 8 AKAP9 A kinase (PRKA) anchor protein (yotiao) 9 AKT1 v-akt murine thymoma viral oncogene homolog 1 AKT2 v-akt murine thymoma viral oncogene homolog 2 AKT3 v-akt murine thymoma viral oncogene homolog 3 ARRB1 arrestin, beta 1 ARRB2 arrestin, beta 2 BRAF v-raf murine sarcoma viral oncogene homolog B1 CACNA1D calcium channel, voltage-dependent, L type, alpha 1D subunit CALM1 calmodulin 1 (phosphorylase kinase, delta) CALM3 calmodulin 3 (phosphorylase kinase, delta) CAMK2A calcium/calmodulin-dependent protein kinase II alpha CAMK2G calcium/calmodulin-dependent protein kinase II gamma CAMK4 calcium/calmodulin-dependent protein kinase IV CLCN6 chloride channel, voltage-sensitive 6 CNGA3 cyclic nucleotide gated channel alpha 3 CNGB1 cyclic nucleotide gated channel beta 1 CRHR1 corticotropin releasing hormone receptor 1 CRHR2 corticotropin releasing hormone receptor 2 GCG glucagon GCGR glucagon receptor GNAI1 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 GNAI2 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 GNAQ guanine nucleotide binding protein (G protein), q polypeptide GNAS GNAS complex locus GNB3 guanine nucleotide binding protein (G protein), beta polypeptide 3 GNG2 guanine nucleotide binding protein (G protein), gamma 2 GNG4 guanine nucleotide binding protein (G protein), gamma 4 HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog ITPR1 inositol 1,4,5-trisphosphate receptor, type 1 KCNK7 potassium channel, subfamily K, member 7 KDR kinase insert domain receptor (a type III receptor tyrosine kinase) KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog MAPK14 mitogen-activated protein kinase 14 MET met proto-oncogene (hepatocyte growth factor receptor) NRAS neuroblastoma RAS viral (v-ras) oncogene homolog NTN1 netrin 1 PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide PIK3CB phosphoinositide-3-kinase, catalytic, beta polypeptide PIK3CD phosphoinositide-3-kinase, catalytic, delta polypeptide PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) PLA2G2A phospholipase A2, group IIA (platelets, synovial fluid) PLA2G4A phospholipase A2, group IVA (cytosolic, calcium- dependent) PLA2G7 phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) PLCG1 phospholipase C, gamma 1 PPP3CA protein phosphatase 3, catalytic subunit, alpha isozyme PRKACA protein kinase, cAMP-dependent, catalytic, alpha PRKACB protein kinase, cAMP-dependent, catalytic, beta PRKAR1A protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) PRKAR2A protein kinase, cAMP-dependent, regulatory, type II, alpha PRKCA protein kinase C, alpha PRKCB protein kinase C, beta PRKCD protein kinase C, delta PRKCE protein kinase C, epsilon PRKCQ protein kinase C, theta RAP1A RAP1A, member of RAS oncogene family RAP2A RAP2A, member of RAS oncogene family RAPGEF3 Rap guanine nucleotide exchange factor (GEF) 3 RAPGEF4 Rap guanine nucleotide exchange factor (GEF) 4 RGS2 regulator of G-protein signaling 2, 24kDa RGS4 regulator of G-protein signaling 4 RPS6KB1 ribosomal protein S6 kinase, 70kDa, polypeptide 1 RYR1 ryanodine receptor 1 (skeletal) RYR2 ryanodine receptor 2 (cardiac) SRC v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) UCN urocortin UTS2 urotensin 2 ACTB Actin, beta B2M Beta-2-microglobulin GAPDH Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK1 Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $495.00 |