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B-Cell Receptor Signaling

The B-Cell Receptor (BCR) signaling pathway involves a complex cascade of events initiated by antigen binding. The signaling pathway begins with the assembly and activation of a crucial molecular complex called the signalosome It is governed by numerous genes that encode the receptor itself, associated signaling molecules, adaptor proteins, kinases, and downstream transcription factors. The core of the BCR complex is the membrane-bound immunoglobulin (which binds the antigen), non-covalently associated with a signaling heterodimer responsible for signal transduction. CD79a molecule contains an ITAM (Immunoreceptor Tyrosine-based Activation Motif) for signal initiation. CD79b molecule contains a second ITAM for signal initiation. Src family tyrosine kinase A phosphorylates the ITAMs on CD79A/B upon antigen binding, initiating the cascade.Other Src-family kinases with similar roles in initial ITAM phosphorylation. Phosphorylated ITAMs recruit the SYK kinase, forming the signalosome, a critical hub that amplifies and branches the signal.

Human B-Cell Receptor Signaling Primer Library
A ready-to-use Human B-Cell Receptor Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against B-cell receptor signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

AKT1 v-akt murine thymoma viral oncogene homolog 1

ATF2 activating transcription factor 2

BCAR1 breast cancer anti-estrogen resistance 1

BCL10 B-cell CLL/lymphoma 10

BCL6 B-cell CLL/lymphoma 6

BLK BLK proto-oncogene, Src family tyrosine kinase

BLNK B-cell linker

BRAF B-Raf proto-oncogene, serine/threonine kinase

BTK Bruton agammaglobulinemia tyrosine kinase

CAMK2A calcium/calmodulin-dependent protein kinase II alpha

CARD10 caspase recruitment domain family, member 10

CBL Cbl proto-oncogene, E3 ubiquitin protein ligase

CD19 CD19 molecule

CD22 CD22 molecule

PTPRC protein tyrosine phosphatase, receptor type C

CD79A CD79a molecule, immunoglobulin-associated alpha

CD79B CD79b molecule, immunoglobulin-associated beta

CD81 CD81 molecule

CDC42 cell division cycle 42

CHUK conserved helix-loop-helix ubiquitous kinase

CR2 complement component (3d/Epstein Barr virus) receptor 2

CREB1 cAMP responsive element binding protein 1

CRK v-crk avian sarcoma virus CT10 oncogene homolog

CRKL v-crk avian sarcoma virus CT10 oncogene homolog-like

ELK1 ELK1, member of ETS oncogene family

ETS1 v-ets avian erythroblastosis virus E26 oncogene homolog 1

FOXO1 forkhead box O1

FYN FYN proto-oncogene, Src family tyrosine kinase

GAB2 GRB2-associated binding protein 2

GRB2 growth factor receptor-bound protein 2

GSK3A glycogen synthase kinase 3 alpha

GSK3B glycogen synthase kinase 3 beta

GTF2I general transcription factor IIi

HCLS1 hematopoietic cell-specific Lyn substrate 1

HRAS Harvey rat sarcoma viral oncogene homolog

IKBKB inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta

IKBKG inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma

ILF2 interleukin enhancer binding factor 2

INPP5D inositol polyphosphate-5-phosphatase, 145kDa

JUN jun proto-oncogene

LCK LCK proto-oncogene, Src family tyrosine kinase

LYN LYN proto-oncogene, Src family tyrosine kinase

MALT1 MALT1 paracaspase

MAP2K1 mitogen-activated protein kinase kinase 1

MAP2K2 mitogen-activated protein kinase kinase 2

MAP2K6 mitogen-activated protein kinase kinase 6

MAP3K7 mitogen-activated protein kinase kinase kinase 7

MAPK1 mitogen-activated protein kinase 1

MAPK14 mitogen-activated protein kinase 14

MAPK3 mitogen-activated protein kinase 3

MAPK4 mitogen-activated protein kinase 4

MAPK8 mitogen-activated protein kinase 8

MAPK9 mitogen-activated protein kinase 9

MAX MYC associated factor X

MYC v-mycavian myelocytomatosis viral oncogene homolog

NCK1 NCK adaptor protein 1

NFATC1 nuclear factor of activated T-cells 1

NFATC2 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2

NFATC3 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3

NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

NFKBIA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

PDPK1 3-phosphoinositide dependent protein kinase 1

PDPK2 PDPK2 protein

PIK3AP1 phosphoinositide-3-kinase adaptor protein 1

PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

PIP5K1A phosphatidylinositol-4-phosphate 5-kinase, type I, alpha

PIP5K1B phosphatidylinositol-4-phosphate 5-kinase, type I, beta

PIP5K1C phosphatidylinositol-4-phosphate 5-kinase, type I, gamma

PLCG1 phospholipase C, gamma 1

PLCG2 phospholipase C, gamma 2 (phosphatidylinositol- specific)

PRKCB protein kinase C beta

PTPN11 protein tyrosine phosphatase, non-receptor type 11

PTPN18 protein tyrosine phosphatase, non-receptor type 18 (brain-derived)

PTPN6 protein tyrosine phosphatase, non-receptor type 6

RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

RAC2 ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)

RAF1 Raf-1 proto-oncogene, serine/threonine kinase

RAPGEF1 Rap guanine nucleotide exchange factor (GEF) 1

RASGRP3 RAS guanyl releasing protein 3 (calcium and DAG- regulated)

RELA v-rel avian reticuloendotheliosis viral oncogene homolog A

RPS6KA1 ribosomal protein S6 kinase, 90kDa, polypeptide 1

SH3BP2 SH3-domain binding protein 2

SHC1 SHC (Src homology 2 domain containing) transforming protein 1

SOS1 son of sevenless homolog 1 (Drosophila)

SYK spleen tyrosine kinase

TEC tecprotein tyrosine kinase

VAV1 vav 1 guanine nucleotide exchange factor

VAV2 vav 2 guanine nucleotide exchange factor

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPD Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a
$495.00
HBCR-I
Mouse B-Cell Receptor Signaling Primer Library
A ready-to-use Mouse B-Cell Receptor Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against B-cell receptor signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

AKT1 v-akt murine thymoma viral oncogene homolog 1

ATF2 activating transcription factor 2

BCAR1 breast cancer anti-estrogen resistance 1

BCL10 B-cell CLL/lymphoma 10

BCL6 B-cell CLL/lymphoma 6

BLK BLK proto-oncogene, Src family tyrosine kinase

BLNK B-cell linker

BRAF B-Raf proto-oncogene, serine/threonine kinase

BTK Bruton agammaglobulinemia tyrosine kinase

CAMK2A calcium/calmodulin-dependent protein kinase II alpha

CARD10 caspase recruitment domain family, member 10

CBL Cbl proto-oncogene, E3 ubiquitin protein ligase

CD19 CD19 molecule

CD22 CD22 molecule

PTPRC protein tyrosine phosphatase, receptor type C

CD79A CD79a molecule, immunoglobulin-associated alpha

CD79B CD79b molecule, immunoglobulin-associated beta

CD81 CD81 molecule

CDC42 cell division cycle 42

CHUK conserved helix-loop-helix ubiquitous kinase

CR2 complement component (3d/Epstein Barr virus) receptor 2

CREB1 cAMP responsive element binding protein 1

CRK v-crk avian sarcoma virus CT10 oncogene homolog

CRKL v-crk avian sarcoma virus CT10 oncogene homolog-like

ELK1 ELK1, member of ETS oncogene family

ETS1 v-ets avian erythroblastosis virus E26 oncogene homolog 1

FOXO1 forkhead box O1

FYN FYN proto-oncogene, Src family tyrosine kinase

GAB2 GRB2-associated binding protein 2

GRB2 growth factor receptor-bound protein 2

GSK3A glycogen synthase kinase 3 alpha

GSK3B glycogen synthase kinase 3 beta

GTF2I general transcription factor IIi

HCLS1 hematopoietic cell-specific Lyn substrate 1

HRAS Harvey rat sarcoma viral oncogene homolog

IKBKB inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta

IKBKG inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma

ILF2 interleukin enhancer binding factor 2

INPP5D inositol polyphosphate-5-phosphatase, 145kDa

JUN jun proto-oncogene

LCK LCK proto-oncogene, Src family tyrosine kinase

LYN LYN proto-oncogene, Src family tyrosine kinase

MALT1 MALT1 paracaspase

MAP2K1 mitogen-activated protein kinase kinase 1

MAP2K2 mitogen-activated protein kinase kinase 2

MAP2K6 mitogen-activated protein kinase kinase 6

MAP3K7 mitogen-activated protein kinase kinase kinase 7

MAPK1 mitogen-activated protein kinase 1

MAPK14 mitogen-activated protein kinase 14

MAPK3 mitogen-activated protein kinase 3

MAPK4 mitogen-activated protein kinase 4

MAPK8 mitogen-activated protein kinase 8

MAPK9 mitogen-activated protein kinase 9

MAX MYC associated factor X

MYC v-myc avian myelocytomatosis viral oncogene homolog

NCK1 NCK adaptor protein 1

NFATC1 nuclear factor of activated T-cells 1

NFATC2 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2

NFATC3 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3

NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

NFKBIA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

PDPK1 3-phosphoinositide dependent protein kinase 1

PDPK2 PDPK2 protein

PIK3AP1 phosphoinositide-3-kinase adaptor protein 1

PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

PIP5K1A phosphatidylinositol-4-phosphate 5-kinase, type I, alpha

PIP5K1B phosphatidylinositol-4-phosphate 5-kinase, type I, beta

PIP5K1C phosphatidylinositol-4-phosphate 5-kinase, type I, gamma

PLCG1 phospholipase C, gamma 1

PLCG2 phospholipase C, gamma 2 (phosphatidylinositol- specific)

PRKCB protein kinase C beta

PTPN11 protein tyrosine phosphatase, non-receptor type 11

PTPN18 protein tyrosine phosphatase, non-receptor type 18 (brain-derived)

PTPN6 protein tyrosine phosphatase, non-receptor type 6

RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

RAC2 ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)

RAF1 Raf-1 proto-oncogene, serine/threonine kinase

RAPGEF1 Rap guanine nucleotide exchange factor (GEF) 1

RASGRP3 RAS guanyl releasing protein 3 (calcium and DAG- regulated)

RELA v-rel avian reticuloendotheliosis viral oncogene homolog A

RPS6KA1 ribosomal protein S6 kinase, 90kDa, polypeptide 1

SH3BP2 SH3-domain binding protein 2

SHC1 SHC (Src homology 2 domain containing) transforming protein 1

SOS1 son of sevenless homolog 1 (Drosophila)

SYK spleen tyrosine kinase

TEC tec protein tyrosine kinase

VAV1 vav 1 guanine nucleotide exchange factor

VAV2 vav 2 guanine nucleotide exchange factor

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPD Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a
$495.00
MBCR-I