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BDNF Signaling

The BDNF signaling pathway is a critical molecular mechanism that regulates key functions in the central nervous system, particularly neuronal survival, differentiation, and synaptic plasticity. BDNF (Brain-Derived Neurotrophic Factor) is a protein growth factor that primarily acts by binding to two types of receptors on the cell surface, leading to distinct cellular outcomes. BDNF's effects are mediated by its binding to a high-affinity receptor and a low-affinity receptor. High-Affinity Receptor: The most important pathway for BDNF's beneficial effects involves the tropomyosin receptor kinase B. When the active form of BDNF (mature BDNF, or m-BDNF) binds to TrkB, the receptor is induced to form a dimer (two receptors join together). This dimerization triggers the activation and autophosphorylation of the intracellular tyrosine kinase domain. Phosphorylated TrkB then acts as a docking site, activating several major intracellular signaling cascades. The MAPK/ERK pathway primarily promotes neuronal differentiation and growth. The PI3K/AKT pathway is crucial for neuronal survival and inhibiting apoptosis (programmed cell death). The PLC gamma pathway is involved in synaptic plasticity and the release of intracellular calcium. Neurogenesis (formation of new neurons), Neuronal survival and growth, Enhancement of synaptic plasticity (essential for learning and memory. Axon and dendrite growth. The Low-Affinity Receptor p75NTRBDNF also binds to the p75 neurotrophin receptor, which generally mediates opposing, or less desirable, effects. The precursor form of BDNF (pro-BDNF) preferentially binds to p75NTRBDNF. This binding can activate pathways that are associated with cellular stress and death. Promotion of neuronal apoptosis (cell death). Facilitation of synaptic Long-Term Depression (LTD), which weakens synaptic connections. It can also act as a co-receptor with TrkB to fine-tune its activity.

Human BDNF Signaling Primer Library
A ready-to-use Human BDNF Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against BDNF signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ACACB acetyl-CoA carboxylase beta

AKT1 v-akt murine thymoma viral oncogene homolog 1

BDNF brain-derived neurotrophic factor

CAMK1 calcium/calmodulin-dependent protein kinase I

CDC42 cell division cycle 42

CDH2 cadherin 2, type 1, N-cadherin (neuronal)

CDK5 cyclin-dependent kinase 5

CDK5R1 cyclin-dependent kinase 5, regulatory subunit 1 (p35)

CEBPB CCAAT/enhancer binding protein (C/EBP), beta

CFL1 cofilin 1 (non-muscle)

CNR1 cannabinoid receptor 1 (brain)

CREB1 cAMP responsive element binding protein 1

CTNNB1 catenin (cadherin-associated protein), beta 1, 88kDa

DLG1 discs, large homolog 1 (Drosophila)

DOCK3 dedicator of cytokinesis 3

DOK5 docking protein 5

ELK1 ELK1, member of ETS oncogene family

FRS2 fibroblast growth factor receptor substrate 2

FYN FYN proto-oncogene, Src family tyrosine kinase

GABRB1 gamma-aminobutyric acid (GABA) A receptor, beta 1

GRB2 growth factor receptor-bound protein 2

GRIA1 glutamate receptor, ionotropic, AMPA 1

GRIA2 glutamate receptor, ionotropic, AMPA 2

GRIA3 glutamate receptor, ionotropic, AMPA 3

GRIN1 glutamate receptor, ionotropic, N-methyl D-aspartate 1

GRIP1 glutamate receptor interacting protein 1

HRAS Harvey rat sarcoma viral oncogene homolog

IRS1 insulin receptor substrate 1

IRS2 insulin receptor substrate 2

JAK2 Janus kinase 2

JUN jun proto-oncogene

LINGO1 leucine rich repeat and Ig domain containing 1

MAP2K1 mitogen-activated protein kinase kinase 1

MAP2K5 mitogen-activated protein kinase kinase 5

MAP3K1 mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase

MAPK1 mitogen-activated protein kinase 1

MAPK10 mitogen-activated protein kinase 10

MAPK14 mitogen-activated protein kinase 14

MAPK3 mitogen-activated protein kinase 3

MAPK8 mitogen-activated protein kinase 8

MAPK9 mitogen-activated protein kinase 9

MEF2C myocyte enhancer factor 2C

NCAM1 neural cell adhesion molecule 1

NCF1 neutrophil cytosolic factor 1

NCF2 neutrophil cytosolic factor 2

NCK1 NCK adaptor protein 1

NCK2 NCK adaptor protein 2

NFATC4 nuclear factor of activated T-cells, cytoplasmic, calcineurin- dependent 4

NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B- cells 1

NGF nerve growth factor (beta polypeptide)

NGFR nerve growth factor receptor

NSF N-ethylmaleimide-sensitive factor

NTF3 neurotrophin 3

NTRK1 neurotrophic tyrosine kinase, receptor, type 1

NTRK2 neurotrophic tyrosine kinase, receptor, type 2

NTRK3 neurotrophic tyrosine kinase, receptor, type 3

PDPK1 3-phosphoinositide dependent protein kinase 1

PIK3CA phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha

PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

PLCG1 phospholipase C, gamma 1

PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit

PRKCD protein kinase C, delta

PTK2B In multiple Geneids

PTPN11 protein tyrosine phosphatase, non-receptor type 11

PTPRF protein tyrosine phosphatase, receptor type, F

RAB3A RAB3A, member RAS oncogene family

RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

RAF1 Raf-1 proto-oncogene, serine/threonine kinase

RANBP9 RAN binding protein 9

RAP1A RAP1A, member of RAS oncogene family

RASGRF1 Ras protein-specific guanine nucleotide-releasing factor 1

RELA v-rel avian reticuloendotheliosis viral oncogene homolog A

RHOG ras homolog family member G

RPS6KA1 ribosomal protein S6 kinase, 90kDa, polypeptide 1

RPS6KA3 ribosomal protein S6 kinase, 90kDa, polypeptide 3

RPS6KA5 ribosomal protein S6 kinase, 90kDa, polypeptide 5

RPS6KB1 ribosomal protein S6 kinase, 70kDa, polypeptide 1

SHC1 SHC (Src homology 2 domain containing) transforming protein 1

SORT1 sortilin 1

SQSTM1 sequestosome 1

SRC SRC proto-oncogene, non-receptor tyrosine kinase

STAT1 signal transducer and activator of transcription 1, 91kDa

STAT3 signal transducer and activator of transcription 3 (acute-phase response factor)

STAT5A signal transducer and activator of transcription 5A

STAT5B signal transducer and activator of transcription 5B

SYN1 synapsin I

TIAM1 T-cell lymphoma invasion and metastasis 1

VAV2 vav 2 guanine nucleotide exchange factor

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPDH Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a
$495.00
HBDNF-I
Mouse Bdnf Signaling Primer Library
A ready-to-use Mouse Bdnf Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against BDNF signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

Acacb acetyl-CoA carboxylase beta

Akt1 v-akt murine thymoma viral oncogene homolog 1

Bdnf brain-derived neurotrophic factor

Camk1 calcium/calmodulin-dependent protein kinase I

Cdc42 cell division cycle 42

Cdh2 cadherin 2, type 1, N-cadherin (neuronal)

Cdk5 cyclin-dependent kinase 5

Cdk5R1 cyclin-dependent kinase 5, regulatory subunit 1 (p35)

Cebpb CCAAT/enhancer binding protein (C/EBP), beta

Cfl1 cofilin 1 (non-muscle)

Cnr1 cannabinoid receptor 1 (brain)

Creb1 cAMP responsive element binding protein 1

Ctnnb1 catenin (cadherin-associated protein), beta 1, 88kDa

Dlg1 discs, large homolog 1 (Drosophila)

Dock3 dedicator of cytokinesis 3

Dok5 docking protein 5

Elk1 ELK1, member of ETS oncogene family

Frs2 fibroblast growth factor receptor substrate 2

Fyn FYN proto-oncogene, Src family tyrosine kinase

Gabrb1 gamma-aminobutyric acid (GABA) A receptor, beta 1

Grb2 growth factor receptor-bound protein 2

Gria1 glutamate receptor, ionotropic, AMPA 1

Gria2 glutamate receptor, ionotropic, AMPA 2

Gria3 glutamate receptor, ionotropic, AMPA 3

Grin1 glutamate receptor, ionotropic, N-methyl D-aspartate 1

Grip1 glutamate receptor interacting protein 1

Hras Harvey rat sarcoma viral oncogene homolog

Irs1 insulin receptor substrate 1

Irs2 insulin receptor substrate 2

Jak2 Janus kinase 2

Jun jun proto-oncogene

Lingo1 leucine rich repeat and Ig domain containing 1

Map2K1 mitogen-activated protein kinase kinase 1

Map2K5 mitogen-activated protein kinase kinase 5

Map3K1 mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase

Mapk1 mitogen-activated protein kinase 1

Mapk10 mitogen-activated protein kinase 10

Mapk14 mitogen-activated protein kinase 14

Mapk3 mitogen-activated protein kinase 3

Mapk8 mitogen-activated protein kinase 8

Mapk9 mitogen-activated protein kinase 9

Mef2C myocyte enhancer factor 2C

Ncam1 neural cell adhesion molecule 1

Ncf1 neutrophil cytosolic factor 1

Ncf2 neutrophil cytosolic factor 2

Nck1 NCK adaptor protein 1

Nck2 NCK adaptor protein 2

Nfatc4 nuclear factor of activated T-cells, cytoplasmic, calcineurin- dependent 4

Nfkb1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Ngf nerve growth factor (beta polypeptide)

Ngfr nerve growth factor receptor

Nsf N-ethylmaleimide-sensitive factor

Ntf3 neurotrophin 3

Ntrk1 neurotrophic tyrosine kinase, receptor, type 1

Ntrk2 neurotrophic tyrosine kinase, receptor, type 2

Ntrk3 neurotrophic tyrosine kinase, receptor, type 3

Pdpk1 3-phosphoinositide dependent protein kinase 1

Pik3Ca phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha

Pik3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Plcg1 phospholipase C, gamma 1

Prkaa1 protein kinase, AMP-activated, alpha 1 catalytic subunit

Prkcd protein kinase C, delta

Ptk2B In multiple Geneids

Ptpn11 protein tyrosine phosphatase, non-receptor type 11

Ptprf protein tyrosine phosphatase, receptor type, F

Rab3A RAB3A, member RAS oncogene family

Rac1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

Raf1 Raf-1 proto-oncogene, serine/threonine kinase

Ranbp9 RAN binding protein 9

Rap1A RAP1A, member of RAS oncogene family

Rasgrf1 Ras protein-specific guanine nucleotide-releasing factor 1

Rela v-rel avian reticuloendotheliosis viral oncogene homolog A

Rhog ras homolog family member G

Rps6Ka1 ribosomal protein S6 kinase, 90kDa, polypeptide 1

Rps6Ka3 ribosomal protein S6 kinase, 90kDa, polypeptide 3

Rps6Ka5 ribosomal protein S6 kinase, 90kDa, polypeptide 5

Rps6Kb1 ribosomal protein S6 kinase, 70kDa, polypeptide 1

Shc1 SHC (Src homology 2 domain containing) transforming protein 1

Sort1 sortilin 1

Sqstm1 sequestosome 1

Src SRC proto-oncogene, non-receptor tyrosine kinase

Stat1 signal transducer and activator of transcription 1, 91kDa

Stat3 signal transducer and activator of transcription 3 (acute-phase response factor) Stat5A signal transducer and activator of transcription 5A

Stat5B signal transducer and activator of transcription 5B

Syn1 synapsin I

Tiam1 T-cell lymphoma invasion and metastasis 1

Vav2 vav 2 guanine nucleotide exchange factor

Actb Actin, beta

B2M Beta-2-microglobulin

Gapdh Glyceraldehyde-3-phosphate dehydrogenase

Gusb Glucuronidase, beta

Hprt1 Hypoxanthine phosphoribosyltransferase 1

Pgk1 Phosphoglycerate kinase 1

Ppia Peptidylprolyl isomerase A

Rpl13A Ribosomal protein L13a
$495.00
MBDNF-I
Rat Bdnf Signaling Primer Library
A ready-to-use Rat Bdnf Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against BDNF signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

Acacb acetyl-CoA carboxylase beta

Akt1 v-akt murine thymoma viral oncogene homolog 1

Bdnf brain-derived neurotrophic factor

Camk1 calcium/calmodulin-dependent protein kinase I

Cdc42 cell division cycle 42

Cdh2 cadherin 2, type 1, N-cadherin (neuronal)

Cdk5 cyclin-dependent kinase 5

Cdk5R1 cyclin-dependent kinase 5, regulatory subunit 1 (p35)

Cebpb CCAAT/enhancer binding protein (C/EBP), beta

Cfl1 cofilin 1 (non-muscle)

Cnr1 cannabinoid receptor 1 (brain)

Creb1 cAMP responsive element binding protein 1

Ctnnb1 catenin (cadherin-associated protein), beta 1, 88kDa

Dlg1 discs, large homolog 1 (Drosophila)

Dock3 dedicator of cytokinesis 3

Dok5 docking protein 5

Elk1 ELK1, member of ETS oncogene family

Frs2 fibroblast growth factor receptor substrate 2

Fyn FYN proto-oncogene, Src family tyrosine kinase

Gabrb1 gamma-aminobutyric acid (GABA) A receptor, beta 1

Grb2 growth factor receptor-bound protein 2

Gria1 glutamate receptor, ionotropic, AMPA 1

Gria2 glutamate receptor, ionotropic, AMPA 2

Gria3 glutamate receptor, ionotropic, AMPA 3

Grin1 glutamate receptor, ionotropic, N-methyl D-aspartate 1

Grip1 glutamate receptor interacting protein 1

Hras Harvey rat sarcoma viral oncogene homolog

Irs1 insulin receptor substrate 1

Irs2 insulin receptor substrate 2

Jak2 Janus kinase 2

Jun jun proto-oncogene

Lingo1 leucine rich repeat and Ig domain containing 1

Map2K1 mitogen-activated protein kinase kinase 1

Map2K5 mitogen-activated protein kinase kinase 5

Map3K1 mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase

Mapk1 mitogen-activated protein kinase 1

Mapk10 mitogen-activated protein kinase 10

Mapk14 mitogen-activated protein kinase 14

Mapk3 mitogen-activated protein kinase 3

Mapk8 mitogen-activated protein kinase 8

Mapk9 mitogen-activated protein kinase 9

Mef2C myocyte enhancer factor 2C

Ncam1 neural cell adhesion molecule 1

Ncf1 neutrophil cytosolic factor 1

Ncf2 neutrophil cytosolic factor 2

Nck1 NCK adaptor protein 1

Nck2 NCK adaptor protein 2

Nfatc4 nuclear factor of activated T-cells, cytoplasmic, calcineurin- dependent 4

Nfkb1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Ngf nerve growth factor (beta polypeptide)

Ngfr nerve growth factor receptor

Nsf N-ethylmaleimide-sensitive factor

Ntf3 neurotrophin 3

Ntrk1 neurotrophic tyrosine kinase, receptor, type 1

Ntrk2 neurotrophic tyrosine kinase, receptor, type 2

Ntrk3 neurotrophic tyrosine kinase, receptor, type 3

Pdpk1 3-phosphoinositide dependent protein kinase 1

Pik3Ca phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha

Pik3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Plcg1 phospholipase C, gamma 1

Prkaa1 protein kinase, AMP-activated, alpha 1 catalytic subunit

Prkcd protein kinase C, delta

Ptk2B In multiple Geneids

Ptpn11 protein tyrosine phosphatase, non-receptor type 11

Ptprf protein tyrosine phosphatase, receptor type, F

Rab3A RAB3A, member RAS oncogene family

Rac1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

Raf1 Raf-1 proto-oncogene, serine/threonine kinase

Ranbp9 RAN binding protein 9

Rap1A RAP1A, member of RAS oncogene family

Rasgrf1 Ras protein-specific guanine nucleotide-releasing factor 1

Rela v-rel avian reticuloendotheliosis viral oncogene homolog A

Rhog ras homolog family member G

Rps6Ka1 ribosomal protein S6 kinase, 90kDa, polypeptide 1

Rps6Ka3 ribosomal protein S6 kinase, 90kDa, polypeptide 3

Rps6Ka5 ribosomal protein S6 kinase, 90kDa, polypeptide 5

Rps6Kb1 ribosomal protein S6 kinase, 70kDa, polypeptide 1

Shc1 SHC (Src homology 2 domain containing) transforming protein 1

Sort1 sortilin 1

Sqstm1 sequestosome 1

Src SRC proto-oncogene, non-receptor tyrosine kinase

Stat1 signal transducer and activator of transcription 1, 91kDa

Stat3 signal transducer and activator of transcription 3 (acute-phase response factor) Stat5A signal transducer and activator of transcription 5A

Stat5B signal transducer and activator of transcription 5B

Syn1 synapsin I

Tiam1 T-cell lymphoma invasion and metastasis 1

Vav2 vav 2 guanine nucleotide exchange factor

Actb Actin, beta

B2M Beta-2-microglobulin

Gapdh Glyceraldehyde-3-phosphate dehydrogenase

Gusb Glucuronidase, beta

Hprt1 Hypoxanthine phosphoribosyltransferase 1

Pgk1 Phosphoglycerate kinase 1

Ppia Peptidylprolyl isomerase A

Rpl13A Ribosomal protein L13a
$595.00
RBDNF-I