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Actin Cytoskeleton Signaling

Actin cytoskeleton signaling is the complex network of biochemical pathways that dynamically control the assembly, disassembly, and organization of the cell's actin filaments (microfilaments). This signaling system allows the cell to rapidly change its shape, motility, and internal structure in response to external and internal stimuli.

The actin cytoskeleton acts as both a target and a transducer of signals, linking information from cell-surface receptors to fundamental cellular actions.

Core Mechanism The primary players in actin signaling are the Rho family of small GTPases:

External Stimuli: Signals from transmembrane receptors—such as Integrins (for adhesion), Receptor Tyrosine Kinases (RTKs), and G protein-coupled receptors (GPCRs)—activate specific Guanosine Exchange Factors (GEFs).

GTPase Activation: The GEFs activate one of the main Rho GTPases: RhoA, Rac1, or Cdc42, by helping them exchange GDP (inactive) for GTP (active).

Downstream Effects: Each activated GTPase then signals to its specific downstream effector proteins, which directly manipulate the actin network to create distinct cellular structures:

Human Actin Cytoskeleton Signaling Primer Library
A ready-to-use Human Actin Cytoskeleton Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against human actin cytoskeleton signaling related genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



Gene List:

ABI2 abl-interactor 2

ACTG1 actin gamma 1

ACTN1 actinin, alpha 1

ARHGAP35 rho GTPase activating protein 35

ARHGEF1 Rho guanine nucleotide exchange factor (GEF) 1

ARHGEF4 Rho guanine nucleotide exchange factor (GEF) 4

ARHGEF6 Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6

ARHGEF7 Rho guanine nucleotide exchange factor (GEF) 7

ARPC5 actin related protein 2/3 complex, subunit 5, 16kDa

BAIAP2 BAI1-associated protein 2

BCAR1 breast cancer anti-estrogen resistance 1

BRAF B-Raf proto-oncogene, serine/threonine kinase

CD14 CD14 molecule

CDC42 cell division cycle 42

CFL1 cofilin 1 (non-muscle)

CFL2 cofilin 2 (muscle)

CHRM1 cholinergic receptor, muscarinic 1

CHRM2 cholinergic receptor, muscarinic 2

CHRM3 cholinergic receptor, muscarinic 3

CHRM4 cholinergic receptor, muscarinic 4

CRK v-crk avian sarcoma virus CT10 oncogene homolog

CSK c-src tyrosine kinase

CYFIP2 cytoplasmic FMR1 interacting protein 2

DIAPH1 diaphanous homolog 1

DIAPH2 diaphanous homolog 2

DOCK1 dedicator of cytokinesis 1

ENAH enabled homolog (Drosophila)

F2 coagulation factor II (thrombin)

F2R coagulation factor II (thrombin) receptor

FGD1 FYVE, RhoGEF and PH domain containing 1

FN1 fibronectin 1

GIT1 G protein-coupled receptor kinase interacting ArfGAP 1

GNA12 guanine nucleotide binding protein (G protein) alpha 12

GNA13 guanine nucleotide binding protein (G protein), alpha 13

GNG12 guanine nucleotide binding protein (G protein), gamma 12

GSN gelsolin

HRAS Harvey rat sarcoma viral oncogene homolog

INS insulin

IQGAP1 IQ motif containing GTPase activating protein 1

ITGA1 integrin, alpha 1

LIMK1 LIM domain kinase 1

MAP2K1 mitogen-activated protein kinase kinase 1

MAP2K2 mitogen-activated protein kinase kinase 2

MAPK1 mitogen-activated protein kinase 1

MAPK3 mitogen-activated protein kinase 3

MAPK4 mitogen-activated protein kinase 4

MAPK6 mitogen-activated protein kinase 6

MOS v-mos Moloney murine sarcoma viral oncogene homolog

MSN moesin

MYL1 myosin, light chain 1, alkali; skeletal, fast

MYL3 myosin, light chain 3, alkali; ventricular, skeletal, slow

MYLK myosin light chain kinase

NCKAP1 NCK-associated protein 1

NRAS neuroblastoma RAS viral (v-ras) oncogene homolog

PAK1 p21 protein (Cdc42/Rac)-activated kinase 1

PAK2 p21 protein (Cdc42/Rac)-activated kinase 2

PAK3 p21 protein (Cdc42/Rac)-activated kinase 3

PAK4 p21 protein (Cdc42/Rac)-activated kinase 4

PAK6 p21 protein (Cdc42/Rac)-activated kinase 6

PAK7 p21 protein (Cdc42/Rac)-activated kinase 7

PFN1 profilin 1

PIP5K1A phosphatidylinositol-4-phosphate 5-kinase, type I, alpha

PIP5K1B phosphatidylinositol-4-phosphate 5-kinase, type I, beta

PIP5K1C phosphatidylinositol-4-phosphate 5-kinase, type I, gamma

PIP5KL1 phosphatidylinositol-4-phosphate 5-kinase-like 1

PPP1R12A protein phosphatase 1, regulatory subunit 12A

PTK2 protein tyrosine kinase 2

PXN paxillin

RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

RAC2 ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)

RAC3 ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3)

RAF1 Raf-1 proto-oncogene, serine/threonine kinase

RDX radixin

RHOA ras homolog family member A

ROCK1 Rho-associated, coiled-coil containing protein kinase 1

ROCK2 Rho-associated, coiled-coil containing protein kinase 2

SLC9A1 solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1

SOS1 son of sevenless homolog 1

SOS2 son of sevenless homolog 2

SSH1 slingshot protein phosphatase 1

SSH2 slingshot protein phosphatase 2

SSH3 slingshot protein phosphatase 3

TMSB4X thymosin beta 4, X-linked

VAV1 vav 1 guanine nucleotide exchange factor

VCL vinculin

VIL1 villin 1

WASF1 WAS protein family, member 1

WASF2 WAS protein family, member 2

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPDH Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

Pgk1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a
$495.00
HACS-I
Mouse Actin Cytoskeleton Signaling Primer Library
A ready-to-use Mouse Actin Cytoskeleton Signaling PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against mouse actin cytoskeleton signaling related genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



Gene List:

ABI2 abl-interactor 2

ACTG1 actin gamma 1

ACTN1 actinin, alpha 1

ARHGAP35 rho GTPase activating protein 35

ARHGEF1 Rho guanine nucleotide exchange factor (GEF) 1

ARHGEF4 Rho guanine nucleotide exchange factor (GEF) 4

ARHGEF6 Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6

ARHGEF7 Rho guanine nucleotide exchange factor (GEF) 7

ARPC5 actin related protein 2/3 complex, subunit 5, 16kDa

BAIAP2 BAI1-associated protein 2

BCAR1 breast cancer anti-estrogen resistance 1

BRAF B-Raf proto-oncogene, serine/threonine kinase

CD14 CD14 molecule

CDC42 cell division cycle 42

CFL1 cofilin 1 (non-muscle)

CFL2 cofilin 2 (muscle)

CHRM1 cholinergic receptor, muscarinic 1

CHRM2 cholinergic receptor, muscarinic 2

CHRM3 cholinergic receptor, muscarinic 3

CHRM4 cholinergic receptor, muscarinic 4

CRK v-crk avian sarcoma virus CT10 oncogene homolog

CSK c-src tyrosine kinase

CYFIP2 cytoplasmic FMR1 interacting protein 2

DIAPH1 diaphanous homolog 1

DIAPH2 diaphanous homolog 2

DOCK1 dedicator of cytokinesis 1

ENAH enabled homolog (Drosophila)

F2 coagulation factor II (thrombin)

F2R coagulation factor II (thrombin) receptor

FGD1 FYVE, RhoGEF and PH domain containing 1

FN1 fibronectin 1

GIT1 G protein-coupled receptor kinase interacting ArfGAP 1

GNA12 guanine nucleotide binding protein (G protein) alpha 12

GNA13 guanine nucleotide binding protein (G protein), alpha 13

GNG12 guanine nucleotide binding protein (G protein), gamma 12

GSN gelsolin

HRAS Harvey rat sarcoma viral oncogene homolog

INS insulin

IQGAP1 IQ motif containing GTPase activating protein 1

ITGA1 integrin, alpha 1

LIMK1 LIM domain kinase 1

MAP2K1 mitogen-activated protein kinase kinase 1

MAP2K2 mitogen-activated protein kinase kinase 2

MAPK1 mitogen-activated protein kinase 1

MAPK3 mitogen-activated protein kinase 3

MAPK4 mitogen-activated protein kinase 4

MAPK6 mitogen-activated protein kinase 6

MOS v-mos Moloney murine sarcoma viral oncogene homolog

MSN moesin

MYL1 myosin, light chain 1, alkali; skeletal, fast

MYL3 myosin, light chain 3, alkali; ventricular, skeletal, slow

MYLK myosin light chain kinase

NCKAP1 NCK-associated protein 1

NRAS neuroblastoma RAS viral (v-ras) oncogene homolog

PAK1 p21 protein (Cdc42/Rac)-activated kinase 1

PAK2 p21 protein (Cdc42/Rac)-activated kinase 2

PAK3 p21 protein (Cdc42/Rac)-activated kinase 3

PAK4 p21 protein (Cdc42/Rac)-activated kinase 4

PAK6 p21 protein (Cdc42/Rac)-activated kinase 6

PAK7 p21 protein (Cdc42/Rac)-activated kinase 7

PFN1 profilin 1

PIP5K1A phosphatidylinositol-4-phosphate 5-kinase, type I, alpha

PIP5K1B phosphatidylinositol-4-phosphate 5-kinase, type I, beta

PIP5K1C phosphatidylinositol-4-phosphate 5-kinase, type I, gamma

PIP5KL1 phosphatidylinositol-4-phosphate 5-kinase-like 1

PPP1R12A protein phosphatase 1, regulatory subunit 12A

PTK2 protein tyrosine kinase 2

PXN paxillin

RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

RAC2 ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)

RAC3 ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3)

RAF1 Raf-1 proto-oncogene, serine/threonine kinase

RDX radixin

RHOA ras homolog family member A

ROCK1 Rho-associated, coiled-coil containing protein kinase 1

ROCK2 Rho-associated, coiled-coil containing protein kinase 2

SLC9A1 solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1

SOS1 son of sevenless homolog 1

SOS2 son of sevenless homolog 2

SSH1 slingshot protein phosphatase 1

SSH2 slingshot protein phosphatase 2

SSH3 slingshot protein phosphatase 3

TMSB4X thymosin beta 4, X-linked

VAV1 vav 1 guanine nucleotide exchange factor

VCL vinculin

VIL1 villin 1

WASF1 WAS protein family, member 1

WASF2 WAS protein family, member 2

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPDH Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

Pgk1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a
$495.00
MACS-I