Necroptosis

Necroptosis is an ordered process of necrotic cell death (necrosis) that is vital to tissue development and homeostasis. Necroptosis requires a variety of genes including RIPK3 and MLKL Induction of necroptosis occurrs through death receptors, interferons, toll-like receptors, intracellular RNA and DNA sensors. For example, the binding of TNF to its receptor induces the formation of a signaling complex during inflammation or during cell survival situations. In some instances, if caspase 8 is inhibited, the necrosome complex forms leading to RIPK1 and RIPK3 activiity leading to necroptosis. Similarly, FASL/TRAIL can induce necroptosis. Stimulation through the TLR receptor complexes by LPS, CD14, MYD88 and other molecules the RHIM-containing adapter TRIF. The Necroptosis PCR Primer Library contains a variety of genes that encompass this emerging cell death pathway and may be utilized to study gene expression using real time PCR arrays.