Human PI3K-AKT Signaling Primer Library

Contains 88 primer sets directed against PI3K/AKT signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ABL1 c-abl oncogene 1, receptor tyrosine kinase

ACACA acetyl-Coenzyme A carboxylase alpha

ACOX1 acyl-Coenzyme A oxidase 1, palmitoyl

AEBP1 AE binding protein 1

AKT1 v-akt murine thymoma viral oncogene homolog 1

AKT2 v-akt murine thymoma viral oncogene homolog 2

AKT3 v-akt murine thymoma viral oncogene homolog 3

ANG angiogenin, ribonuclease, RNase A family, 5

ARAF v-raf murine sarcoma 3611 viral oncogene homolog

BAD BCL2-associated agonist of cell death

BCL2 B-cell CLL/lymphoma 2

BCL2L1 BCL2-like 1

BRAF v-raf murine sarcoma viral oncogene homolog B1

BTK Bruton agammaglobulinemia tyrosine kinase

CAP1 CAP, adenylate cyclase-associated protein 1 (yeast)

CASP9 caspase 9, apoptosis-related cysteine peptidase

CBL Cas-Br-M (murine) ecotropic retroviral transforming sequence

CCND1 cycline D1

CCR2 chemokine (C-C motif) receptor 2

CCR5 chemokine (C-C motif) receptor 5

CD4 CD4 molecule

CD40 CD40 molecule, TNF receptor superfamily member 5

CDC42 cell division cycle 42 (GTP binding protein, 25kDa)

CDH1 cadherin 1, type 1, E-cadherin (epithelial)

CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1)

CDKN1B cyclin-dependent kinase inhibitor 1B (p27, Kip1)

CDKN2A cyclin-dependent kinase inhibitor 2A (p16)

CREB1 cAMP responsive element binding protein 1

CTNNB1 catenin (cadherin-associated protein), beta 1, 88kDa

CXCR4 chemokine (C-X-C motif) receptor 4

E2F1 E2F transcription factor 1

EGFR epidermal growth factor receptor

ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2

FRAP1 Fkbp12-Rapamycin Complex-Associated Protein 1

GRB2 growth factor receptor-bound protein 2

GSK3A glycogen synthase kinase 3 alpha

GSK3B glycogen synthase kinase 3 beta

GYS1 glycogen synthase 1 (muscle)

HK2 hexokinase 2

HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog

IGF1 insulin-like growth factor 1 (somatomedin C)

IGF1R insulin-like growth factor 1 receptor

IGF2 insulin-like growth factor 2 (somatomedin A)

IGF2R insulin-like growth factor 2 receptor

INS insulin

INSR insulin receptor

IRS1 insulin receptor substrate 1

IRS2 insulin receptor substrate 2

IRS4 insulin receptor substrate 4

ITGB1 integrin, beta 1

ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)

JAK2 Janus kinase 2

KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene

KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

LCK lymphocyte-specific protein tyrosine kinase

LEP leptin

MAP2K1 mitogen-activated protein kinase kinase 1

MAPK1 mitogen-activated protein kinase 1

MAPK14 mitogen-activated protein kinase 14

MAPK8 mitogen-activated protein kinase 8

MYC v-myc myelocytomatosis viral oncogene homolog (avian)

NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

NFKBIA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

NOS3 nitric oxide synthase 3 (endothelial cell)

PAK1 p21 protein (Cdc42/Rac)-activated kinase 1

PDE3B phosphodiesterase 3B, cGMP-inhibited

PDPK1 3-phosphoinositide dependent protein kinase-1

PIK3C2B phosphoinositide-3-kinase, class 2, beta polypeptide

PIK3C3 phosphoinositide-3-kinase, class 3

PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide

PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

PIK3R4 phosphoinositide-3-kinase, regulatory subunit 4

PRKCA protein kinase C, alpha

PRKCE protein kinase C, epsilon

PRKCG protein kinase C, gamma

PTEN phosphatase and tensin homolog

RAC1 ras-related C3 botulinum toxin substrate 1

RAF1 v-raf-1 murine leukemia viral oncogene homolog 1

RELA v-rel reticuloendotheliosis viral oncogene homolog A (avian)

RHOA ras homolog gene family, member A

SGK1 serum/glucocorticoid regulated kinase 1

SHC1 SHC (Src homology 2 domain containing) transforming protein 1

SOS1 son of sevenless homolog 1 (Drosophila)

SPP1 secreted phosphoprotein 1

SRC v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

SYK spleen tyrosine kinase

TLR4 toll-like receptor 4

TNF tumor necrosis factor (TNF superfamily, member 2)

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPD Glyceraldehyde phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a