Human Notch Signaling Pathway

Contains 88 primer sets directed against NOTCH signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:



ADAM17 ADAM metallopeptidase domain 17

AKT1 v-akt murine thymoma viral oncogene homolog 1

APH1A anterior pharynx defective 1 homolog A (C. elegans)

APH1B anterior pharynx defective 1 homolog B (C. elegans)

CCND1 Cyclin D1

CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1)

CIR1 corepressor interacting with RBPJ, 1

CREBBP CREB binding protein

CTBP1 C-terminal binding protein 1

CTBP2 C-terminal binding protein 2

CUL1 cullin 1

DLL1 delta-like 1 (Drosophila)

DLL3 delta-like 3 (Drosophila)

DLL4 delta-like 4 (Drosophila)

DTX1 deltex homolog 1 (Drosophila)

DTX2 deltex homolog 2 (Drosophila)

DTX3 deltex homolog 3 (Drosophila)

DTX3L deltex 3-like (Drosophila)

DTX4 deltex homolog 4 (Drosophila)

DVL1 dishevelled, dsh homolog 1 (Drosophila)

DVL2 dishevelled, dsh homolog 2 (Drosophila)

DVL3 dishevelled, dsh homolog 3 (Drosophila)

EP300 E1A binding protein p300

FBXW7 F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase

FHL1 four and a half LIM domains 1

GATA3 GATA binding protein 3

GSK3B glycogen synthase kinase 3 beta

HAT1 histone acetyltransferase 1

HDAC1 histone deacetylase 1

HDAC2 histone deacetylase 2

HDAC3 histone deacetylase 3

HDAC4 histone deacetylase 4

HDAC5 histone deacetylase 5

HDAC6 histone deacetylase 6

HDAC7 histone deacetylase 7

HDAC8 histone deacetylase 8

HDAC9 histone deacetylase 9

HDAC10 histone deacetylase 10

HDAC11 histone deacetylase 11

HES1 hairy and enhancer of split 1

HES5 hairy and enhancer of split 5

HES6 hairy and enhancer of split 6

HEY1 hairy/enhancer-of-split related with YRPW motif 1

HEY2 hairy/enhancer-of-split related with YRPW motif 2

INPP5K Inositol polyphosphate-5-phosphatase K

ITCH itchy E3 ubiquitin protein ligase

JAG1 jagged 1

JAG2 jagged 2

JAK2 Janus kinase 2

KAT2A K(lysine) acetyltransferase 2A

KAT2B K(lysine) acetyltransferase 2B

LCK lymphocyte-specific protein tyrosine kinase

LFNG LFNG O-fucosylpeptide 3-beta-N- acetylglucosaminyltransferase

MAGEA1 melanoma antigen family A, 1 (directs expression of antigen MZ2-E)

MAML1 mastermind-like 1 (Drosophila)

MAML2 mastermind-like 2 (Drosophila)

MAML3 mastermind-like 3 (Drosophila)

MFNG MFNG O-fucosylpeptide 3-beta-N- acetylglucosaminyltransferase

MTOR mechanistic target of rapamycin (serine/threonine kinase)

MYC V-myc myelocytomatosis viral oncogene homolog

NCOR1 nuclear receptor corepressor 1

NCOR2 nuclear receptor corepressor 2

NCSTN nicastrin

NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

NOTCH1 notch 1

NOTCH2 notch 2

NOTCH2NL Notch homolog 2 (Drosophila) N-terminal like

NOTCH3 notch 3

NOTCH4 notch 4

NUMB numb homolog (Drosophila)

NUMBL numb homolog (Drosophila)-like

PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

PIK3R2 phosphoinositide-3-kinase, regulatory subunit 2 (beta)

PSEN1 presenilin 1

PSEN2 presenilin 2

PSENEN presenilin enhancer 2 homolog (C. elegans)

PTCRA pre T-cell antigen receptor alpha

RBPJ recombination signal binding protein for immunoglobulin kappa J region

RFNG RFNG O-fucosylpeptide 3-beta-N- acetylglucosaminyltransferase

RING1 ring finger protein 1

SAP30 Sin3A-associated protein, 30kDa

SKP1 S-phase kinase-associated protein 1

SNW1 SNW domain containing 1

SPEN spen homolog, transcriptional regulator (Drosophila)

SRC v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

STAT3 signal transducer and activator of transcription 3 (acute-phase response factor)

TLE1 transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)

TNF tumor necrosis factor

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPDH Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a